Anti-Influenza A Nucleoprotein Antibody, clone A1 ZooMAb® Mouse Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone A1 is a ZooMAb® mouse recombinant monoclonal antibody that detects Nucleoprotein N1 of Influenza A virus.
• Immunogen: Influenza A virus
• Application: Quality Control Testing Evaluated by Immunocytochemistry in MDCK cells infected with Influenza A virus.Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Influenza A Nucleoprotein in MDCK cells infected with Influenza A virus.Tested applicationsELISA Analysis: 1.0 μg/mL from a representative lot detected recombinant Influenza A virus Nucleoprotein.Affinity Binding Assay: A representative lot of this antibody bound recombinant Influenza A Nucleoprotein with a KD of 2.8 x 10-6 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Nucleoprotein (UniProt: P03466; also known as Nucleocapsid protein, Protein N) is encoded by the NP gene (Gene ID: 956531) in Influenza A virus. Influenza A viruses belong to the Orthomyxoviridae family and encapsidates the negative strand viral RNA that protects it from the action of nucleases. Its envelope membrane is reported to carry hemagglutinin, neuraminidase, and the ion channel protein M2. Inside the membrane there are layers of matrix protein 1 and ribonucleoprotein (RNP) complexes that contain viral RNA and binding proteins. RNP serves as template for transcription and replication and must be localized in the host cell nucleus to start an infectious cycle. However, due to its large size, RNP cannot diffuse through the nuclear pore complex and is transported through CRM-1-dependent nuclear export machinery. Viral nucleoprotein contains two nuclear localization signals (NLS) that are responsible for active RNP import into the nucleus. It has been reported that M1 interaction with RNP hides nucleoprotein′s NLS and soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. This dissociation of M1 from RNP unmasks nucleoprotein′s NLS, targeting the RNP to the nucleus. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.