Anti-hnRNP A1 Antibody, clone 9H10 ZooMAb® Mouse Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 9H10 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detects Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1).
• Immunogen: Full-length, native, purified human Heterogeneous nuclear ribonucleoprotein A1.
• Application: Quality Control TestingEvaluated by Western Blotting in NIH3T3 cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected hnRNP A1 in NIH3T3 cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected hnRNP A1 in lysates from HEK293, HeLa, and C6 cells.Flow Cytometry Analysis: 0.1 μg from a representative lot detected hnRNP A1 in one million HepG2 cells.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected hnRNP A1 in A431, HeLa, and NIH3T3 cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected hnRNP A1 in human pancreas tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Heterogeneous nuclear ribonucleoprotein A1 (UniProt: P09651; also known as Nuclear ribonucleoprotein particle A1 protein) is encoded by the HNRNPA1 gene (Gene ID: 3178) in human. hnRNAP A1 is a member of the hnRNP family of conserved proteins that is involved in RNA transcription, pre-mRNA splicing, mRNA transport, protein translation, microRNA processing, telomere maintenance, and the regulation of transcription factor activity. It is ubiquitously expressed, however, due to post-translational modifications it can vary in its molecular function. It is mainly localized in cytoplasmic mRNP granules containing untranslated mRNAs and shuttles continuously between the nucleus and the cytoplasm along with mRNA. Its N-terminal region contains two RNA recognition motifs (aa 14-97 and 105-184) and the C-terminal region has the prion-like domain (PrLD) that mediates cellular compartmentalization, protein-protein interaction, and RNA-binding. The RRMs are composed of four β-sheets adjoined by two -helices (β1 1β2β3 2β4).The β-sheet surfaces primarily interact with RNA via amino acid stretches containing predominantly aromatic residues. hnRNP A1 is considered a key component of the response to cellular stresses and also binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1. Three isoforms of hnRNP A1 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Clarke, JP., et al. (2021). Front. Mol. Biosci. 8; 659610; Li, ML., et al. (2019). PLoS One. 14(9); e0221048).
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.