Anti-MLL/HRX CT Antibody, clone 5C15 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 5C15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Histone-lysine N-methyltransferase 2A (MLL/HRX). It targets an epitope within 21 amino acids from the MLL cleavage product C180 region.
• Immunogen: KLH-conjugated linear peptide corresponding to 21 amino acids from the p180 C-terminal subunit of human Histone-lysine N-methyltransferase 2A (MLL/HRX).
• Application: Quality Control TestingEvaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected MLL/HRX in human kidney tissue sections.Tested ApplicationsAffinity Binding Assay: A representative lot of this antibody bound MLL peptides with a KD of 1.2 x 10-6 in an affinity binding assay.Immunocytochemistry Analysis: A 1:100 dilution from representative lot detected MLL/HRX CT in HeLa and A431 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Histone-lysine N-methyltransferase 2A (UniProt: Q03164; also known as EC:2.1.1.354, Lysine N-methyltransferase 2A, ALL-1, CXXC-type zinc finger protein 7, Myeloid/lymphoid or mixed-lineage leukemia, Myeloid/lymphoid or mixed-lineage leukemia protein 1, MLL, Trithorax-like protein, Zinc finger protein HRX) is encoded by the KMT2A (also known as ALL1, CXXC7, HRX, HTRX, MLL, MLL1, TRX1) gene (Gene ID: 4297) in human. MLL is a histone methyltransferase that plays an essential role in early development and hematopoiesis. It is expressed in heart, lung, brain, and in T and B lymphocytes. Catalytic subunit of the MLL complex mediates both methylation of lysine 4 of histone H3 (H3K4me) complex and acetylation of lysine 16 of histone H4 (H4K16ac). By itself it has weak methyltransferase activity and requires another component of the MLL complex to obtain full methyltransferase activity. MLL does not display any activity towards histone H3 phosphorylated on threonine 3 and has less activity towards histone H3 dimethylated on arginine 8 and lysine 9. However, it displays high activity towards histone H3 acetylated on lysine 9. MLL is normally cleaved into a N-terminal p320 and a C-terminal p180 cleavage product. This cleavage is brought about by taspase 1 (TASP1) at cleavage site 1 (aa 2666-2667) and cleavage site 2 (aa 2718-2719). The p320 and p180 units can heterodimerize via SET and FYRC domains. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.