Anti-GSDMC Antibody, clone 2H7 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2H7 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Gasdermin-C (GSDMC). It targets an epitope within 21 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human Gasdermin-C (GSDMC).
• Application: Quality Control TestingEvaluated by Western Blotting in Rat kidney tissue lysate.Western Blotting Analysis: A 1,000 dilution of this antibody detected GSDMC in Rat kidney tissue lysate.Tested ApplicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GSDMC in Human kidney tissue sections.Affinity Binding Assay:: A representative lot of this antibody bound GSDMC peptide with a KD of 3.1 x 10-9 in an affinity binding assay.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected GSDMC in HepG2 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Gasdermin-C (UniProt: Q9BYG8; also known as Melanoma-derived leucine zipper-containing extranuclear factor, GSDMC) is encoded by the GSDMC (also known as MLZE) gene (Gene ID: 56169) in human. Gasdermin-C is a pyroptosis executioner that is identified in cancer cells and is reported to facilitate inflammatory tumor death. Its expression is observed mainly in trachea and spleen. Following its synthesis, it is cleaved into N-terminal (aa 1-240) and C-terminal (aa 241-508) fragments. The cleavage of Gasdermin-C occurs by caspase-8 in response to macrophage-secreted TNF- . The full-length protein before cleavage is shown to be inactive and intramolecular interactions between N- and C-terminal domains mediate autoinhibition in the absence of activation signal. Upon cleavage, the N-terminal domain is liberated and undergoes oligomerization and translocates to the inner leaflet lipids in the membrane where it forms pores leading to pyroptosis. It is reported to sensitize tumor cells to PARP inhibitors in tumor models. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hou, J., et al. (2023). Methods Mol Biol. 2641; 135-146; Zhang, J-Y., et al. (2021). Cell Res. 31(9); 980-997; Hou, J., et al. (2020). Nat. Cell Biol. 22(10); 1264-1275).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.