17-101-25MI

MilliporeSigma™ Upstate™ ChIPAb+™ Dimethyl-Histone H3 (Lys79)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Manufacturer: MilliporeSigma™

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Pack Size SKU Availability Price
Each of 1 17-101-25MI-Each-of-1 In Stock ₹ 52,449.99

17-101-25MI - Each of 1

₹ 52,449.99

In Stock

Quantity

1

Base Price: ₹ 52,449.99

GST (18%): ₹ 9,440.998

Total Price: ₹ 61,890.988

Antigen

ChIPAb+™ Dimethyl-Histone H3 (Lys79)α

Classification

Monoclonal

Formulation

Anti-Dimethyl-Histone H3 (Lys79) (rabbit Monoclonal), One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Negative Control Supernatant, One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA

Gene Symbols

H3F3A, H3F3B

Immunogen

KLH-conjugated synthetic peptide containing the sequence DFme2KTD corresponding to dimethyl-lysine at position 79 of histone H3. The immunizing sequence is identical in human, mouse, rat and yeast. A C-terminal cysteine was added to facilitate conjugation.

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Recognizes dimethyl-histone H3 (Lys79).

Target Species

Human, Vertebrates

Form

Culture Supernatant

Applications

ChIP Assay, Western Blot

Conjugate

Unconjugated

Gene Accession No.

Q16695

Host Species

Rabbit

Quantity

25 Assays

Research Discipline

Epigenetics, Nuclear Function

Gene ID (Entrez)

NM_003493

Includes

This ChIPAb+ Dimethyl-Histone H3 (Lys79) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage

-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Isotype

IgG

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Description

  • Specifically detects ChIPAb+™ Dimethyl-Histone H3 (Lys79)α Clone: in Human, Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells
  • Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure
  • The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes
  • These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

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MilliporeSigma™

17-101-25MI

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Antigen:
ChIPAb+™ Dimethyl-Histone H3 (Lys79)α

Classification:
Monoclonal

Formulation:
Anti-Dimethyl-Histone H3 (Lys79) (rabbit Monoclonal), One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Negative Control Supernatant, One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA

Gene Symbols:
H3F3A, H3F3B

Immunogen:
KLH-conjugated synthetic peptide containing the sequence DFme2KTD corresponding to dimethyl-lysine at position 79 of histone H3. The immunizing sequence is identical in human, mouse, rat and yeast. A C-terminal cysteine was added to facilitate conjugation.

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes dimethyl-histone H3 (Lys79).

Target Species:
Human, Vertebrates

Form:
Culture Supernatant

Applications:
ChIP Assay, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
Q16695

Host Species:
Rabbit

Quantity:
25 Assays

Research Discipline:
Epigenetics, Nuclear Function

Gene ID (Entrez):
NM_003493

Includes:
This ChIPAb+ Dimethyl-Histone H3 (Lys79) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Isotype:
IgG

Img

MilliporeSigma™

17-101-30MI

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Antigen:
Trimethyl-Histone H3 (Lys79)

Classification:
Polyclonal

Formulation:
Anti-Trimethyl-Histone H3 (Lys79) (rabbit polyclonal). One vial containing 25μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150mM NaCl) with 0.05% sodium azide before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA

Gene Symbols:
HIST3H3, H3FT, H3t, H3T, H3/g, H3.4, H3/t

Immunogen:
Linear Peptide corresponding to human Histone H3 methylated at Lys79.

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This antibody recognizes Histone H3 Trimethylated on Lys79.

Target Species:
Human, Vertebrates

Form:
__

Applications:
Multiplex, Immunoassay, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
Q16695

Host Species:
Rabbit

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_003484.1

Includes:
This ChIPAb+ Trimethyl-Histone H3 (Lys79) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Isotype:
__

Img

MilliporeSigma™

17-101-31MI

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Antigen:
Phospho-CREB (Ser133)

Classification:
Polyclonal

Formulation:
Anti-phospho-CREB (Ser133) (rabbit polyclonal). One vial containing 100μg of affinity purified rabbit serum in 0.1M Tris-Glycine (pH 7.4) 0.15M NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers cFos CRE. One vial containing 75μL of 5μM of each primer specific for the cFos CRE. FOR: GGC CCA CGA GAC CTC TGA GAC A; REV: GCC TTG GCG CGT GTC CTA ATC T

Gene Symbols:
CREB1, CREB, MGC9284

Immunogen:
KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes p43 phosphorylated CREB. May also recognize p30 and p38kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.

Target Species:
Hamster, Human, Mouse, Rat

Form:
Purified

Applications:
ChIP Assay, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
P16220

Host Species:
Rabbit

Quantity:
25 Assays

Research Discipline:
Signaling, Epigenetics and Nuclear Function

Gene ID (Entrez):
NM_134442

Includes:
This ChIPAb+ Phospho-CREB (Ser133) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Isotype:
IgG

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17-101-35MI

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Antigen:
CBX8

Classification:
Polyclonal

Formulation:
Anti-CBX8 (Rabbit Polyclonal). One vial containing 50μg affinity purified rabbit polyclonal in 72μL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, HOXA2 Upstream. One vial containing 75μL of 5μM of each primer specific for human HOXA2 promoter region (chr7:27142948-27143085, hg19 build). FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C

Gene Symbols:
HPC3, PC3, Pc3, RC1, hPc3

Immunogen:
__

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
__

Target Species:
Human

Form:
Purified

Applications:
ChIP Assay, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
Q9HC52

Host Species:
Rabbit

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_065700

Includes:
Use ChIPAb+ CBX8 ChIP Validated rabbit polyclonal antibody for the detection of CBX8 also known as Chromobox protein homolog 8 and Polycomb 3 homolog.

Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Isotype:
__