17-101-30MI
MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys79), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 17-101-30MI-Each-of-1 | In Stock | ₹ 51,079.32 |
17-101-30MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 51,079.32
GST (18%): ₹ 9,194.278
Total Price: ₹ 60,273.598
Antigen
Trimethyl-Histone H3 (Lys79)
Classification
Polyclonal
Formulation
Anti-Trimethyl-Histone H3 (Lys79) (rabbit polyclonal). One vial containing 25μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150mM NaCl) with 0.05% sodium azide before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Symbols
HIST3H3, H3FT, H3t, H3T, H3/g, H3.4, H3/t
Immunogen
Linear Peptide corresponding to human Histone H3 methylated at Lys79.
Quantity
25 Assays
Research Discipline
Epigenetics and Nuclear Function
Gene ID (Entrez)
NP_003484.1
Includes
This ChIPAb+ Trimethyl-Histone H3 (Lys79) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications
Multiplex, Immunoassay, Western Blot
Conjugate
Unconjugated
Gene Accession No.
Q16695
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This antibody recognizes Histone H3 Trimethylated on Lys79.
Target Species
Human, Vertebrates
Related Products
Description
- Specifically detects Trimethyl-Histone H3 (Lys79) Clone: in Human, Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Multiplexing, Western Blotting Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin
- The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each)
- Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin
- Histone modifications regulate DNA transcription, repair, recombination, and replication
- The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination
- Lysine methylation occurs in three distinct states, having either one (me1), two (me2) or three (me3) methyl groups attached to the amine group of the lysine side chain
- Methylation of Lysine 79 in Histone 3 acts as a marker of inactive chromatin regions
- Methylation by Dot1 methylase on lysine-79 (Lys79) of Histone H3 is criticial for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.
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Antigen: Trimethyl-Histone H3 (Lys79)
Classification: Polyclonal
Formulation: Anti-Trimethyl-Histone H3 (Lys79) (rabbit polyclonal). One vial containing 25μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150mM NaCl) with 0.05% sodium azide before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Symbols: HIST3H3, H3FT, H3t, H3T, H3/g, H3.4, H3/t
Immunogen: Linear Peptide corresponding to human Histone H3 methylated at Lys79.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_003484.1
Includes: This ChIPAb+ Trimethyl-Histone H3 (Lys79) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: Multiplex, Immunoassay, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q16695
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This antibody recognizes Histone H3 Trimethylated on Lys79.
Target Species: Human, Vertebrates
Antigen:
Trimethyl-Histone H3 (Lys79)
Classification:
Polyclonal
Formulation:
Anti-Trimethyl-Histone H3 (Lys79) (rabbit polyclonal). One vial containing 25μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150mM NaCl) with 0.05% sodium azide before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Symbols:
HIST3H3, H3FT, H3t, H3T, H3/g, H3.4, H3/t
Immunogen:
Linear Peptide corresponding to human Histone H3 methylated at Lys79.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_003484.1
Includes:
This ChIPAb+ Trimethyl-Histone H3 (Lys79) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
Multiplex, Immunoassay, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q16695
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This antibody recognizes Histone H3 Trimethylated on Lys79.
Target Species:
Human, Vertebrates
Antigen: Phospho-CREB (Ser133)
Classification: Polyclonal
Formulation: Anti-phospho-CREB (Ser133) (rabbit polyclonal). One vial containing 100μg of affinity purified rabbit serum in 0.1M Tris-Glycine (pH 7.4) 0.15M NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers cFos CRE. One vial containing 75μL of 5μM of each primer specific for the cFos CRE. FOR: GGC CCA CGA GAC CTC TGA GAC A; REV: GCC TTG GCG CGT GTC CTA ATC T
Gene Symbols: CREB1, CREB, MGC9284
Immunogen: KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.
Quantity: 25 Assays
Research Discipline: Signaling, Epigenetics and Nuclear Function
Gene ID (Entrez): NM_134442
Includes: This ChIPAb+ Phospho-CREB (Ser133) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Gene Accession No.: P16220
Host Species: Rabbit
Purification Method: Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes p43 phosphorylated CREB. May also recognize p30 and p38kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.
Target Species: Hamster, Human, Mouse, Rat
Antigen:
Phospho-CREB (Ser133)
Classification:
Polyclonal
Formulation:
Anti-phospho-CREB (Ser133) (rabbit polyclonal). One vial containing 100μg of affinity purified rabbit serum in 0.1M Tris-Glycine (pH 7.4) 0.15M NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers cFos CRE. One vial containing 75μL of 5μM of each primer specific for the cFos CRE. FOR: GGC CCA CGA GAC CTC TGA GAC A; REV: GCC TTG GCG CGT GTC CTA ATC T
Gene Symbols:
CREB1, CREB, MGC9284
Immunogen:
KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.
Quantity:
25 Assays
Research Discipline:
Signaling, Epigenetics and Nuclear Function
Gene ID (Entrez):
NM_134442
Includes:
This ChIPAb+ Phospho-CREB (Ser133) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
P16220
Host Species:
Rabbit
Purification Method:
Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes p43 phosphorylated CREB. May also recognize p30 and p38kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.
Target Species:
Hamster, Human, Mouse, Rat
Antigen: CBX8
Classification: Polyclonal
Formulation: Anti-CBX8 (Rabbit Polyclonal). One vial containing 50μg affinity purified rabbit polyclonal in 72μL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, HOXA2 Upstream. One vial containing 75μL of 5μM of each primer specific for human HOXA2 promoter region (chr7:27142948-27143085, hg19 build). FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Gene Symbols: HPC3, PC3, Pc3, RC1, hPc3
Immunogen: __
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_065700
Includes: Use ChIPAb+ CBX8 ChIP Validated rabbit polyclonal antibody for the detection of CBX8 also known as Chromobox protein homolog 8 and Polycomb 3 homolog.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q9HC52
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: __
Target Species: Human
Antigen:
CBX8
Classification:
Polyclonal
Formulation:
Anti-CBX8 (Rabbit Polyclonal). One vial containing 50μg affinity purified rabbit polyclonal in 72μL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, HOXA2 Upstream. One vial containing 75μL of 5μM of each primer specific for human HOXA2 promoter region (chr7:27142948-27143085, hg19 build). FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Gene Symbols:
HPC3, PC3, Pc3, RC1, hPc3
Immunogen:
__
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_065700
Includes:
Use ChIPAb+ CBX8 ChIP Validated rabbit polyclonal antibody for the detection of CBX8 also known as Chromobox protein homolog 8 and Polycomb 3 homolog.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q9HC52
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Target Species:
Human
Antigen: Dimethyl-Histone H3 (Lys36)
Classification: Polyclonal
Formulation: Anti-Dimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 25μL rabbit polyclonal serum containing 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit Serum. One vial containing 25μL antiserum containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. (chr12:6647453+6647539, hg 19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA GC 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’
Gene Symbols: H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Immunogen: KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 dimethylated at Lys36.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_003484
Includes: This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, Electromobility Shift Assay, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q16695
Host Species: Rabbit
Purification Method: __
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes Dimethyl Histone H3 (Lys36). No detectable cross-reactivity unmethylated, mono, or dimethylated Lys36. No detectable cross reactivity to other histone modifications as measured by AbSurance Histone Antibody Specificity Arrays.
Target Species: Human, Vertebrates
Antigen:
Dimethyl-Histone H3 (Lys36)
Classification:
Polyclonal
Formulation:
Anti-Dimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 25μL rabbit polyclonal serum containing 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit Serum. One vial containing 25μL antiserum containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. (chr12:6647453+6647539, hg 19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA GC 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’
Gene Symbols:
H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Immunogen:
KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 dimethylated at Lys36.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_003484
Includes:
This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, Electromobility Shift Assay, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q16695
Host Species:
Rabbit
Purification Method:
__
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes Dimethyl Histone H3 (Lys36). No detectable cross-reactivity unmethylated, mono, or dimethylated Lys36. No detectable cross reactivity to other histone modifications as measured by AbSurance Histone Antibody Specificity Arrays.
Target Species:
Human, Vertebrates