MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys36), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 17-104-93MI-Each-of-1 | In Stock | ₹ 53,561.42 |
17-104-93MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 53,561.42
GST (18%): ₹ 9,641.056
Total Price: ₹ 63,202.476
Antigen
Trimethyl-Histone H3 (Lys36)
Classification
Polyclonal
Formulation
Anti-Trimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 50μL (0.05mg/mL) purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 7.5μg Rabbit IgG in 75μL storage buffer containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region (chr12:6647453+6647539 hg19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA G 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’
Gene Symbols
H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Immunogen
Linear peptide corresponding to human Histone H3 trimethylated at Lys36 with peptide sequence APATGGV(K)KPHRYRPGC
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes Trimethyl-Histone H3 (Lys36), Mr ∽17kDa.
Target Species
Human, Mouse
Form
Purified
Applications
ChIP Assay, ChIP sequencing (ChIP-seq), Dot Blot, Western Blot
Conjugate
Unconjugated
Gene Accession No.
Q16695
Host Species
Rabbit
Quantity
25 Assays
Research Discipline
Epigenetics and Nuclear Function
Gene ID (Entrez)
NP_003484
Includes
This ChIPAb+ Trimethyl-Histone H3 (Lys36) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Related Products
Description
- Specifically detects Trimethyl-Histone H3 (Lys36) Clone: in Human, Mouse samples, and it is validated for ChIP-seq, Chromatin Immunoprecipitation, Dot Blot, Western Blotting Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin
- The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each)
- Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin
- Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm
- These modifications regulate DNA transcription, repair, recombination, and replication
- The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination
- These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the histone code hypothesis)
- The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle
- Methylation of histone H3 on Lys36 (H3K36me2/3) is tightly associated with actively transcribed genes, and this modification is found primarily within the coding region, suggesting H3K36 methylation is necessary for efficient RNA polymerase II elongation and processivity.
Compare Similar Items
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Antigen: Trimethyl-Histone H3 (Lys36)
Classification: Polyclonal
Formulation: Anti-Trimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 50μL (0.05mg/mL) purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 7.5μg Rabbit IgG in 75μL storage buffer containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region (chr12:6647453+6647539 hg19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA G 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’
Gene Symbols: H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Immunogen: Linear peptide corresponding to human Histone H3 trimethylated at Lys36 with peptide sequence APATGGV(K)KPHRYRPGC
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes Trimethyl-Histone H3 (Lys36), Mr ∽17kDa.
Target Species: Human, Mouse
Form: Purified
Applications: ChIP Assay, ChIP sequencing (ChIP-seq), Dot Blot, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q16695
Host Species: Rabbit
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_003484
Includes: This ChIPAb+ Trimethyl-Histone H3 (Lys36) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Antigen: Monomethyl-Histone H3 (Lys36)
Classification: Polyclonal
Formulation: Anti-Monomethyl-Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 75μL 0.35mg/mL purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. FOR: GCC ATG TAG ACC CCT TGA AGA G; REV: ACT GGT TGA GCA CAG GGT ACT TTA T
Gene Symbols: HIST3H3, H3FT, H3/t, H3t, H3/g, H3.4, H3T
Immunogen: KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 monomethylated at Lys36.
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This antibody recognizes the N-terminus of Histone H3 monomethylated at Lys36.
Target Species: Human, Mouse, Rat
Form: __
Applications: ChIP Assay, Dot Blot, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q16695
Host Species: Rabbit
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_003484
Includes: The ChIPAb+ Monomethyl-Histone H3 (Lys36) set includes the monomethyl-Histone H3 (Lys36) antibody, a Normal Rabbit IgG and control primers which amplify a 213bp region of the human GAPDH coding region.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Antigen: __
Classification: __
Formulation: __
Gene Symbols: __
Immunogen: __
Regulatory Status: __
Primary or Secondary: __
Test Specificity: __
Target Species: __
Form: __
Applications: __
Conjugate: __
Gene Accession No.: __
Host Species: __
Quantity: __
Research Discipline: __
Gene ID (Entrez): __
Includes: __
Content And Storage: __
