MilliporeSigma™ Upstate™ Dimethyl-Histone H3 (Lys36), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 17-101-36MI-Each-of-1 | In Stock | ₹ 53,561.42 |
17-101-36MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 53,561.42
GST (18%): ₹ 9,641.056
Total Price: ₹ 63,202.476
Antigen
Dimethyl-Histone H3 (Lys36)
Classification
Polyclonal
Formulation
Anti-Dimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 25μL rabbit polyclonal serum containing 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit Serum. One vial containing 25μL antiserum containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. (chr12:6647453+6647539, hg 19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA GC 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’
Gene Symbols
H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Immunogen
KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 dimethylated at Lys36.
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes Dimethyl Histone H3 (Lys36). No detectable cross-reactivity unmethylated, mono, or dimethylated Lys36. No detectable cross reactivity to other histone modifications as measured by AbSurance Histone Antibody Specificity Arrays.
Target Species
Human, Vertebrates
Form
Serum
Applications
ChIP Assay, Electromobility Shift Assay, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Gene Accession No.
Q16695
Host Species
Rabbit
Quantity
25 Assays
Research Discipline
Epigenetics and Nuclear Function
Gene ID (Entrez)
NP_003484
Includes
This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
Content And Storage
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Related Products
Description
- Specifically detects Dimethyl-Histone H3 (Lys36) Clone: in Human, Vertebrate samples, and it is validated for ChIP, Electromobility Shift Assay, Immunoprecipitation, Western Blotting Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin
- The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each)
- Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin
- Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm
- These modifications regulate DNA transcription, repair, recombination, and replication
- The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination
- These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the histone code hypothesis)
- The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle
- Methylation of histone H3 on Lys36 (H3K36me2/3) is tightly associated with actively transcribed genes, and this modification is found primarily within the coding region, suggesting H3K36 methylation is necessary for efficient RNA polymerase II elongation and processivity.
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Antigen: Dimethyl-Histone H3 (Lys36)
Classification: Polyclonal
Formulation: Anti-Dimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 25μL rabbit polyclonal serum containing 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit Serum. One vial containing 25μL antiserum containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. (chr12:6647453+6647539, hg 19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA GC 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’
Gene Symbols: H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Immunogen: KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 dimethylated at Lys36.
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes Dimethyl Histone H3 (Lys36). No detectable cross-reactivity unmethylated, mono, or dimethylated Lys36. No detectable cross reactivity to other histone modifications as measured by AbSurance Histone Antibody Specificity Arrays.
Target Species: Human, Vertebrates
Form: Serum
Applications: ChIP Assay, Electromobility Shift Assay, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q16695
Host Species: Rabbit
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_003484
Includes: This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Antigen: ChIPAb+™ Phospho-Histone H3 (Thr3)α
Classification: Monoclonal
Formulation: Anti-Phospho-Histone H3 (Thr3) (rabbit monoclonal). One vial containing 50μL of cultured supernantant in 0.05% sodium azide. Negative Control Supernatant. One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Control Primers, human GAPDH promoter. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Symbols: HIST3H3, H3FT, MGC126886, H3t, MGC126888, H3.4, H3T, H3/g, H3/t
Immunogen: Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Histone H3 phosphorylated at Threonine 3; cross-reactivity with phospho-histone H3 Thr22 detected in some applications
Target Species: Human
Form: Culture Supernatant
Applications: ChIP Assay, Dot Blot, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q16695
Host Species: Rabbit
Quantity: 25 Assays
Research Discipline: Epigenetics, Nuclear Function
Gene ID (Entrez): NM_003493
Includes: This ChIPAb+ Phospho-Histone H3 (Thr3) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Antigen: __
Classification: __
Formulation: __
Gene Symbols: __
Immunogen: __
Regulatory Status: __
Primary or Secondary: __
Test Specificity: __
Target Species: __
Form: __
Applications: __
Conjugate: __
Gene Accession No.: __
Host Species: __
Quantity: 1 Vial (minimum of 3 x 10E8 IFU/mL)
Research Discipline: __
Gene ID (Entrez): __
Includes: TagRFP-LC3 Lentivirus: One vial containing 25μL of lentiviral particles at a minimum of 3 x 10E8 infectious units (IFU) per mL; Promoter: EF-1 (Elongation Factor-1)
Content And Storage: Lentivirus is stable for at least 4 months from date of receipt when stored at -80°C.
