17-100-50MI

MilliporeSigma™ Upstate™ Acetyl-Histone H3 (Lys4), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Manufacturer: MilliporeSigma™

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Pack Size SKU Availability Price
Each of 1 17-100-50MI-Each-of-1 In Stock ₹ 52,449.99

17-100-50MI - Each of 1

₹ 52,449.99

In Stock

Quantity

1

Base Price: ₹ 52,449.99

GST (18%): ₹ 9,440.998

Total Price: ₹ 61,890.988

Antigen

Acetyl-Histone H3 (Lys4)

Classification

Polyclonal

Formulation

Anti-Acetyl-Histone H3 (Lys4) (rabbit polyclonal). One vial containing 100μL of immunoaffinity purified rabbit polyclonal in storage buffer containing 0.05% sodium azide with 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA

Gene Symbols

H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945

Immunogen

KLH-conjugated, synthetic peptide containing the sequence …RTAcKQ… in which AcK corresponds to acetyl lysine 4 of human histone H3.

Quantity

25 Assays

Research Discipline

Epigenetics and Nuclear Function

Gene ID (Entrez)

NP_003484

Includes

This ChIPAb+ Acetyl-Histone H3 (Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage

-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Applications

ChIP Assay, Western Blot

Conjugate

Unconjugated

Gene Accession No.

Q16695

Host Species

Rabbit

Purification Method

Affinity Purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Recognizes Histone H3 acetylated on Lys4.

Target Species

Human

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Description

  • Specifically detects Acetyl-Histone H3 (Lys4) Clone: in Human samples, and it is validated for ChIP, Western Blotting Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells
  • Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure
  • Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones
  • The term Histone H3 alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state
  • Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

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MilliporeSigma™

17-100-50MI

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Antigen:
Acetyl-Histone H3 (Lys4)

Classification:
Polyclonal

Formulation:
Anti-Acetyl-Histone H3 (Lys4) (rabbit polyclonal). One vial containing 100μL of immunoaffinity purified rabbit polyclonal in storage buffer containing 0.05% sodium azide with 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA

Gene Symbols:
H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945

Immunogen:
KLH-conjugated, synthetic peptide containing the sequence …RTAcKQ… in which AcK corresponds to acetyl lysine 4 of human histone H3.

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_003484

Includes:
This ChIPAb+ Acetyl-Histone H3 (Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Applications:
ChIP Assay, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
Q16695

Host Species:
Rabbit

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes Histone H3 acetylated on Lys4.

Target Species:
Human

Img

MilliporeSigma™

17-100-51MI

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Antigen:
Acetyl-Histone H3 (Lys14)α

Classification:
Monoclonal

Formulation:
Anti-Acetyl-Histone H3 (Lys14) (rabbit monoclonal). One vial containing 50μL of unpurified Monoclonal in buffer containing 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, with 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Nornal Rabbit IgG. One vial containing 75μL of normal rabbit IgG. ChIP Primers, c-myc promoter. One vial containing 75μL of 5μM of each primer specific for human c-myc promoter. FOR: CCC AAA AAA AGG CAC GGA A; REV: TAT TGG AAA TGC GGT CAT GC

Gene Symbols:
H3.3A, H3.3B, H3F3, MGC87782, MGC87783, OTTHUMP00000035618, OTTHUMP00000035619, OTTHUMP00000035621

Immunogen:
Acetyl-specific synthetic peptide corres-ponding to residues around Lys14 of Histone H3.

Quantity:
25 Assays

Research Discipline:
Epigenetics, Nuclear Function

Gene ID (Entrez):
NP_003484.1

Includes:
This ChIPAb+ Acetyl-Histone H3 (Lys14) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Applications:
ChIP sequencing (ChIP-seq), Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
P84243

Host Species:
Rabbit

Purification Method:
Unpurified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes Histone H3 acetylated on Lys14.

Target Species:
Human, Rat

Img

MilliporeSigma™

17-100-57MI

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Antigen:
SMRTα

Classification:
Monoclonal

Formulation:
Anti-SMRT (mouse monoclonal). One vial containing 50μL of unpurified mouse monoclonal ascites with 0.05% sodium azide. Negative Ascites (mouse). One vial containing 50μL of mouse ascites with 0.05% sodium azide. ChIP Primers, IκBα promoter. One vial containing 75μL of 5μM of each primer specific for human IĸBα promoter. FOR: GAC GAC CCC AAT TCA AAT CG; REV: TCA GGC TCG GGG AAT TTC C

Gene Symbols:
NCOR2, CTG26, N-CoR2, SMAP270, SMRT, SMRTE, SMRTE-tau, TNRC14, TRAC, TRAC-1, TRAC1

Immunogen:
Recombinant protein corresponding to human SMRT.

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_001070729.1

Includes:
The ChIPAb+ SMRT set includes the SMRT antibody, a negative control mouse ascites and qPCR primers which amplify a 299bp region of human IκBα promoter.

Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Applications:
ChIP Assay, Electromobility Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
Q9Y618

Host Species:
Mouse

Purification Method:
Unpurified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes SMRT.

Target Species:
Human, Mouse, Rat

Img

MilliporeSigma™

17-100-61MI

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Antigen:
E2F-1α

Classification:
Monoclonal

Formulation:
Anti-E2F-1 (mouse monoclonal). One vial containing 25μg of protein G purified monoclonal mixed IgG in 25μL of buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution. Nornal Mouse IgG. One vial containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, CDC2 promoter. One vial containing 75μL of 5μM each primer specific for human CDC2 promoter. FOR: CGC CCT TTC CTC TTT CTT TC; REV: ATC GGG TAG CCC GTA GAC TT

Gene Symbols:
E2F1, RBAP-1, E2F-1, RBP3, RBBP3, RBBP-3, PBR3

Immunogen:
GST fusion protein corresponding to full-length human E2F-1.

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_005216

Includes:
This ChIPAb+ E2F-1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Applications:
ChIP Assay, ChIP sequencing (ChIP-seq), Immunoprecipitation

Conjugate:
Unconjugated

Gene Accession No.:
Q01094

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
The epitopes have been mapped to amino acids 1 to 89 and to amino acids 342 to 386.

Target Species:
Human, Xenopus species