17-100-51MI
MilliporeSigma™ Upstate™ Acetyl-Histone H3 (Lys14)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
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Antigen
Acetyl-Histone H3 (Lys14)α
Classification
Monoclonal
Formulation
Anti-Acetyl-Histone H3 (Lys14) (rabbit monoclonal). One vial containing 50μL of unpurified Monoclonal in buffer containing 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, with 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Nornal Rabbit IgG. One vial containing 75μL of normal rabbit IgG. ChIP Primers, c-myc promoter. One vial containing 75μL of 5μM of each primer specific for human c-myc promoter. FOR: CCC AAA AAA AGG CAC GGA A; REV: TAT TGG AAA TGC GGT CAT GC
Gene Symbols
H3.3A, H3.3B, H3F3, MGC87782, MGC87783, OTTHUMP00000035618, OTTHUMP00000035619, OTTHUMP00000035621
Immunogen
Acetyl-specific synthetic peptide corres-ponding to residues around Lys14 of Histone H3.
Quantity
25 Assays
Research Discipline
Epigenetics, Nuclear Function
Gene ID (Entrez)
NP_003484.1
Includes
This ChIPAb+ Acetyl-Histone H3 (Lys14) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications
ChIP sequencing (ChIP-seq), Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Gene Accession No.
P84243
Host Species
Rabbit
Purification Method
Unpurified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes Histone H3 acetylated on Lys14.
Target Species
Human, Rat
Isotype
IgG
Related Products
Description
- Specifically detects Acetyl-Histone H3 (Lys14)α Clone: in Human, Rat samples, and it is validated for ChIP, ChIP-seq, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blotting Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells
- Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure
- The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes
- These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
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Antigen: Acetyl-Histone H3 (Lys14)α
Classification: Monoclonal
Formulation: Anti-Acetyl-Histone H3 (Lys14) (rabbit monoclonal). One vial containing 50μL of unpurified Monoclonal in buffer containing 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, with 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Nornal Rabbit IgG. One vial containing 75μL of normal rabbit IgG. ChIP Primers, c-myc promoter. One vial containing 75μL of 5μM of each primer specific for human c-myc promoter. FOR: CCC AAA AAA AGG CAC GGA A; REV: TAT TGG AAA TGC GGT CAT GC
Gene Symbols: H3.3A, H3.3B, H3F3, MGC87782, MGC87783, OTTHUMP00000035618, OTTHUMP00000035619, OTTHUMP00000035621
Immunogen: Acetyl-specific synthetic peptide corres-ponding to residues around Lys14 of Histone H3.
Quantity: 25 Assays
Research Discipline: Epigenetics, Nuclear Function
Gene ID (Entrez): NP_003484.1
Includes: This ChIPAb+ Acetyl-Histone H3 (Lys14) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP sequencing (ChIP-seq), Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Gene Accession No.: P84243
Host Species: Rabbit
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes Histone H3 acetylated on Lys14.
Target Species: Human, Rat
Isotype: IgG
Antigen:
Acetyl-Histone H3 (Lys14)α
Classification:
Monoclonal
Formulation:
Anti-Acetyl-Histone H3 (Lys14) (rabbit monoclonal). One vial containing 50μL of unpurified Monoclonal in buffer containing 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, with 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Nornal Rabbit IgG. One vial containing 75μL of normal rabbit IgG. ChIP Primers, c-myc promoter. One vial containing 75μL of 5μM of each primer specific for human c-myc promoter. FOR: CCC AAA AAA AGG CAC GGA A; REV: TAT TGG AAA TGC GGT CAT GC
Gene Symbols:
H3.3A, H3.3B, H3F3, MGC87782, MGC87783, OTTHUMP00000035618, OTTHUMP00000035619, OTTHUMP00000035621
Immunogen:
Acetyl-specific synthetic peptide corres-ponding to residues around Lys14 of Histone H3.
Quantity:
25 Assays
Research Discipline:
Epigenetics, Nuclear Function
Gene ID (Entrez):
NP_003484.1
Includes:
This ChIPAb+ Acetyl-Histone H3 (Lys14) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP sequencing (ChIP-seq), Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
P84243
Host Species:
Rabbit
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes Histone H3 acetylated on Lys14.
Target Species:
Human, Rat
Isotype:
IgG
Antigen: SMRTα
Classification: Monoclonal
Formulation: Anti-SMRT (mouse monoclonal). One vial containing 50μL of unpurified mouse monoclonal ascites with 0.05% sodium azide. Negative Ascites (mouse). One vial containing 50μL of mouse ascites with 0.05% sodium azide. ChIP Primers, IκBα promoter. One vial containing 75μL of 5μM of each primer specific for human IĸBα promoter. FOR: GAC GAC CCC AAT TCA AAT CG; REV: TCA GGC TCG GGG AAT TTC C
Gene Symbols: NCOR2, CTG26, N-CoR2, SMAP270, SMRT, SMRTE, SMRTE-tau, TNRC14, TRAC, TRAC-1, TRAC1
Immunogen: Recombinant protein corresponding to human SMRT.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_001070729.1
Includes: The ChIPAb+ SMRT set includes the SMRT antibody, a negative control mouse ascites and qPCR primers which amplify a 299bp region of human IκBα promoter.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, Electromobility Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q9Y618
Host Species: Mouse
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes SMRT.
Target Species: Human, Mouse, Rat
Isotype: IgG2a κ
Antigen:
SMRTα
Classification:
Monoclonal
Formulation:
Anti-SMRT (mouse monoclonal). One vial containing 50μL of unpurified mouse monoclonal ascites with 0.05% sodium azide. Negative Ascites (mouse). One vial containing 50μL of mouse ascites with 0.05% sodium azide. ChIP Primers, IκBα promoter. One vial containing 75μL of 5μM of each primer specific for human IĸBα promoter. FOR: GAC GAC CCC AAT TCA AAT CG; REV: TCA GGC TCG GGG AAT TTC C
Gene Symbols:
NCOR2, CTG26, N-CoR2, SMAP270, SMRT, SMRTE, SMRTE-tau, TNRC14, TRAC, TRAC-1, TRAC1
Immunogen:
Recombinant protein corresponding to human SMRT.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_001070729.1
Includes:
The ChIPAb+ SMRT set includes the SMRT antibody, a negative control mouse ascites and qPCR primers which amplify a 299bp region of human IκBα promoter.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, Electromobility Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q9Y618
Host Species:
Mouse
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes SMRT.
Target Species:
Human, Mouse, Rat
Isotype:
IgG2a κ
Antigen: E2F-1α
Classification: Monoclonal
Formulation: Anti-E2F-1 (mouse monoclonal). One vial containing 25μg of protein G purified monoclonal mixed IgG in 25μL of buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution. Nornal Mouse IgG. One vial containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, CDC2 promoter. One vial containing 75μL of 5μM each primer specific for human CDC2 promoter. FOR: CGC CCT TTC CTC TTT CTT TC; REV: ATC GGG TAG CCC GTA GAC TT
Gene Symbols: E2F1, RBAP-1, E2F-1, RBP3, RBBP3, RBBP-3, PBR3
Immunogen: GST fusion protein corresponding to full-length human E2F-1.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_005216
Includes: This ChIPAb+ E2F-1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, ChIP sequencing (ChIP-seq), Immunoprecipitation
Conjugate: Unconjugated
Gene Accession No.: Q01094
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: The epitopes have been mapped to amino acids 1 to 89 and to amino acids 342 to 386.
Target Species: Human, Xenopus species
Isotype: IgG
Antigen:
E2F-1α
Classification:
Monoclonal
Formulation:
Anti-E2F-1 (mouse monoclonal). One vial containing 25μg of protein G purified monoclonal mixed IgG in 25μL of buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution. Nornal Mouse IgG. One vial containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, CDC2 promoter. One vial containing 75μL of 5μM each primer specific for human CDC2 promoter. FOR: CGC CCT TTC CTC TTT CTT TC; REV: ATC GGG TAG CCC GTA GAC TT
Gene Symbols:
E2F1, RBAP-1, E2F-1, RBP3, RBBP3, RBBP-3, PBR3
Immunogen:
GST fusion protein corresponding to full-length human E2F-1.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_005216
Includes:
This ChIPAb+ E2F-1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, ChIP sequencing (ChIP-seq), Immunoprecipitation
Conjugate:
Unconjugated
Gene Accession No.:
Q01094
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
The epitopes have been mapped to amino acids 1 to 89 and to amino acids 342 to 386.
Target Species:
Human, Xenopus species
Isotype:
IgG
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