17-101-31MI
MilliporeSigma™ Upstate™ Phospho-CREB (Ser133), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 17-101-31MI-Each-of-1 | In Stock | ₹ 52,449.99 |
17-101-31MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 52,449.99
GST (18%): ₹ 9,440.998
Total Price: ₹ 61,890.988
Antigen
Phospho-CREB (Ser133)
Classification
Polyclonal
Formulation
Anti-phospho-CREB (Ser133) (rabbit polyclonal). One vial containing 100μg of affinity purified rabbit serum in 0.1M Tris-Glycine (pH 7.4) 0.15M NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers cFos CRE. One vial containing 75μL of 5μM of each primer specific for the cFos CRE. FOR: GGC CCA CGA GAC CTC TGA GAC A; REV: GCC TTG GCG CGT GTC CTA ATC T
Gene Symbols
CREB1, CREB, MGC9284
Immunogen
KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.
Quantity
25 Assays
Research Discipline
Signaling, Epigenetics and Nuclear Function
Gene ID (Entrez)
NM_134442
Includes
This ChIPAb+ Phospho-CREB (Ser133) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype
IgG
Applications
ChIP Assay, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Gene Accession No.
P16220
Host Species
Rabbit
Purification Method
Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes p43 phosphorylated CREB. May also recognize p30 and p38kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.
Target Species
Hamster, Human, Mouse, Rat
Form
Purified
Related Products
Description
- Specifically detects Phospho-CREB (Ser133) Clone: in Hamster, Human, Mouse, Rat samples, and it is validated for ChIP, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blotting CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements
- CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses
- While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system
- CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations
- CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways
- CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners
- CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.
Compare Similar Items
Show Difference
Antigen: Phospho-CREB (Ser133)
Classification: Polyclonal
Formulation: Anti-phospho-CREB (Ser133) (rabbit polyclonal). One vial containing 100μg of affinity purified rabbit serum in 0.1M Tris-Glycine (pH 7.4) 0.15M NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers cFos CRE. One vial containing 75μL of 5μM of each primer specific for the cFos CRE. FOR: GGC CCA CGA GAC CTC TGA GAC A; REV: GCC TTG GCG CGT GTC CTA ATC T
Gene Symbols: CREB1, CREB, MGC9284
Immunogen: KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.
Quantity: 25 Assays
Research Discipline: Signaling, Epigenetics and Nuclear Function
Gene ID (Entrez): NM_134442
Includes: This ChIPAb+ Phospho-CREB (Ser133) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: IgG
Applications: ChIP Assay, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Gene Accession No.: P16220
Host Species: Rabbit
Purification Method: Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes p43 phosphorylated CREB. May also recognize p30 and p38kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.
Target Species: Hamster, Human, Mouse, Rat
Form: Purified
Antigen:
Phospho-CREB (Ser133)
Classification:
Polyclonal
Formulation:
Anti-phospho-CREB (Ser133) (rabbit polyclonal). One vial containing 100μg of affinity purified rabbit serum in 0.1M Tris-Glycine (pH 7.4) 0.15M NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers cFos CRE. One vial containing 75μL of 5μM of each primer specific for the cFos CRE. FOR: GGC CCA CGA GAC CTC TGA GAC A; REV: GCC TTG GCG CGT GTC CTA ATC T
Gene Symbols:
CREB1, CREB, MGC9284
Immunogen:
KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.
Quantity:
25 Assays
Research Discipline:
Signaling, Epigenetics and Nuclear Function
Gene ID (Entrez):
NM_134442
Includes:
This ChIPAb+ Phospho-CREB (Ser133) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
IgG
Applications:
ChIP Assay, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
P16220
Host Species:
Rabbit
Purification Method:
Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes p43 phosphorylated CREB. May also recognize p30 and p38kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.
Target Species:
Hamster, Human, Mouse, Rat
Form:
Purified
Antigen: CBX8
Classification: Polyclonal
Formulation: Anti-CBX8 (Rabbit Polyclonal). One vial containing 50μg affinity purified rabbit polyclonal in 72μL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, HOXA2 Upstream. One vial containing 75μL of 5μM of each primer specific for human HOXA2 promoter region (chr7:27142948-27143085, hg19 build). FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Gene Symbols: HPC3, PC3, Pc3, RC1, hPc3
Immunogen: __
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_065700
Includes: Use ChIPAb+ CBX8 ChIP Validated rabbit polyclonal antibody for the detection of CBX8 also known as Chromobox protein homolog 8 and Polycomb 3 homolog.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: __
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q9HC52
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: __
Target Species: Human
Form: Purified
Antigen:
CBX8
Classification:
Polyclonal
Formulation:
Anti-CBX8 (Rabbit Polyclonal). One vial containing 50μg affinity purified rabbit polyclonal in 72μL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, HOXA2 Upstream. One vial containing 75μL of 5μM of each primer specific for human HOXA2 promoter region (chr7:27142948-27143085, hg19 build). FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Gene Symbols:
HPC3, PC3, Pc3, RC1, hPc3
Immunogen:
__
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_065700
Includes:
Use ChIPAb+ CBX8 ChIP Validated rabbit polyclonal antibody for the detection of CBX8 also known as Chromobox protein homolog 8 and Polycomb 3 homolog.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
__
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q9HC52
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Target Species:
Human
Form:
Purified
Antigen: Dimethyl-Histone H3 (Lys36)
Classification: Polyclonal
Formulation: Anti-Dimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 25μL rabbit polyclonal serum containing 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit Serum. One vial containing 25μL antiserum containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. (chr12:6647453+6647539, hg 19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA GC 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’
Gene Symbols: H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Immunogen: KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 dimethylated at Lys36.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_003484
Includes: This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: __
Applications: ChIP Assay, Electromobility Shift Assay, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q16695
Host Species: Rabbit
Purification Method: __
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes Dimethyl Histone H3 (Lys36). No detectable cross-reactivity unmethylated, mono, or dimethylated Lys36. No detectable cross reactivity to other histone modifications as measured by AbSurance Histone Antibody Specificity Arrays.
Target Species: Human, Vertebrates
Form: Serum
Antigen:
Dimethyl-Histone H3 (Lys36)
Classification:
Polyclonal
Formulation:
Anti-Dimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 25μL rabbit polyclonal serum containing 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit Serum. One vial containing 25μL antiserum containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. (chr12:6647453+6647539, hg 19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA GC 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’
Gene Symbols:
H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Immunogen:
KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 dimethylated at Lys36.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_003484
Includes:
This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
__
Applications:
ChIP Assay, Electromobility Shift Assay, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q16695
Host Species:
Rabbit
Purification Method:
__
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes Dimethyl Histone H3 (Lys36). No detectable cross-reactivity unmethylated, mono, or dimethylated Lys36. No detectable cross reactivity to other histone modifications as measured by AbSurance Histone Antibody Specificity Arrays.
Target Species:
Human, Vertebrates
Form:
Serum
Antigen: ChIPAb+™ Phospho-Histone H3 (Thr3)α
Classification: Monoclonal
Formulation: Anti-Phospho-Histone H3 (Thr3) (rabbit monoclonal). One vial containing 50μL of cultured supernantant in 0.05% sodium azide. Negative Control Supernatant. One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Control Primers, human GAPDH promoter. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Symbols: HIST3H3, H3FT, MGC126886, H3t, MGC126888, H3.4, H3T, H3/g, H3/t
Immunogen: Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
Quantity: 25 Assays
Research Discipline: Epigenetics, Nuclear Function
Gene ID (Entrez): NM_003493
Includes: This ChIPAb+ Phospho-Histone H3 (Thr3) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: __
Applications: ChIP Assay, Dot Blot, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q16695
Host Species: Rabbit
Purification Method: __
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Histone H3 phosphorylated at Threonine 3; cross-reactivity with phospho-histone H3 Thr22 detected in some applications
Target Species: Human
Form: Culture Supernatant
Antigen:
ChIPAb+™ Phospho-Histone H3 (Thr3)α
Classification:
Monoclonal
Formulation:
Anti-Phospho-Histone H3 (Thr3) (rabbit monoclonal). One vial containing 50μL of cultured supernantant in 0.05% sodium azide. Negative Control Supernatant. One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Control Primers, human GAPDH promoter. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Symbols:
HIST3H3, H3FT, MGC126886, H3t, MGC126888, H3.4, H3T, H3/g, H3/t
Immunogen:
Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
Quantity:
25 Assays
Research Discipline:
Epigenetics, Nuclear Function
Gene ID (Entrez):
NM_003493
Includes:
This ChIPAb+ Phospho-Histone H3 (Thr3) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
__
Applications:
ChIP Assay, Dot Blot, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q16695
Host Species:
Rabbit
Purification Method:
__
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Histone H3 phosphorylated at Threonine 3; cross-reactivity with phospho-histone H3 Thr22 detected in some applications
Target Species:
Human
Form:
Culture Supernatant