17-105-31MI

MilliporeSigma™ Upstate™ LSD1 (KDM1A), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Manufacturer: MilliporeSigma™

Select a Size

Pack Size SKU Availability Price
Each of 1 17-105-31MI-Each-of-1 In Stock ₹ 53,561.42

17-105-31MI - Each of 1

₹ 53,561.42

In Stock

Quantity

1

Base Price: ₹ 53,561.42

GST (18%): ₹ 9,641.056

Total Price: ₹ 63,202.476

Antigen

LSD1 (KDM1A)

Classification

Polyclonal

Formulation

Anti-LSD1 (Rabbit Polyclonal). One vial containing 50μg affinity purified rabbit polyclonal in 72μL buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide, before the addition of glycerol to 30%. Final concentration of antibody 0.7mg/mL. Normal rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, PSA. One vial containing 75μL of 5μM of each primer specific for human prostate-specific antigen (PSA) enhancer region (chr19:51354179-51354263, hg19 build). FOR: GCC TGG ATC TGA GAG AGA TAT CAT C; REV: ACA CCT TTT TTT TTC TGG ATT GTT G

Gene Symbols

KDM1A, AOF2, KDM1, LSD1

Immunogen

Histidine-tagged recombinant protein corresponding to full length LSD1.

Quantity

25 Assays

Research Discipline

Epigenetics and Nuclear Function

Gene ID (Entrez)

NP_055828

Target Species

Human, Mouse, Rat

Applications

ChIP Assay, Immunoprecipitation, Western Blot

Conjugate

Unconjugated

Gene Accession No.

O60341

Host Species

Rabbit

Purification Method

Affinity Purified

Regulatory Status

RUO

Primary or Secondary

Primary

Includes

Use ChIPAb+ LSD1 (KDM1A) ChIP Validated mouse monoclonal antibody for the detection of LSD1 (Lysine-specific histone demethylase 1A) also known as BRAF35-HDAC complex protein BHC110, includes the antibody and the specific control PCR primers.

Content And Storage

-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

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Description

  • Specifically detects LSD1 (KDM1A) Clone: in Human, Mouse, Rat samples, and it is validated for ChIP, Immunoprecipitation, Western Blotting LSD1 (lysine specific demethylase 1), also known as BHC110, is a histone demethylase that specifically demethylates Lys4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor
  • LSD1 acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed
  • LSD1 demethylates both mono- and tri-methylted Lys4 of histone H3, and may play a role in the repression of neuronal genes
  • Alone, it is unable to demethylate H3 Lys4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity
  • LSD1 may also demethylate Lys9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

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MilliporeSigma™

17-105-31MI

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Antigen:
LSD1 (KDM1A)

Classification:
Polyclonal

Formulation:
Anti-LSD1 (Rabbit Polyclonal). One vial containing 50μg affinity purified rabbit polyclonal in 72μL buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide, before the addition of glycerol to 30%. Final concentration of antibody 0.7mg/mL. Normal rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, PSA. One vial containing 75μL of 5μM of each primer specific for human prostate-specific antigen (PSA) enhancer region (chr19:51354179-51354263, hg19 build). FOR: GCC TGG ATC TGA GAG AGA TAT CAT C; REV: ACA CCT TTT TTT TTC TGG ATT GTT G

Gene Symbols:
KDM1A, AOF2, KDM1, LSD1

Immunogen:
Histidine-tagged recombinant protein corresponding to full length LSD1.

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_055828

Target Species:
Human, Mouse, Rat

Applications:
ChIP Assay, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
O60341

Host Species:
Rabbit

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Includes:
Use ChIPAb+ LSD1 (KDM1A) ChIP Validated mouse monoclonal antibody for the detection of LSD1 (Lysine-specific histone demethylase 1A) also known as BRAF35-HDAC complex protein BHC110, includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Img

MilliporeSigma™

17-105-32MI

--


Antigen:
PCAF

Classification:
Polyclonal

Formulation:
Anti-PCAF (Rabbit Polyclonal). One vial containing 17.5μg purified rabbit polyclonal in 25μL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of 30% glycerol (0.7mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, PRAME. One vial containing 75μL of 5μM of each primer specific for human preferentially expressed antigen in melanoma (chr22:22901678+22901804, hg19 build). FOR: GAA GCC TCG CCG GAA CTC; REV: TCC TCC CCA TCT CTG CAG AA

Gene Symbols:
GCN5, GCN5L, P/CAF, P, CAF, PCAF, KAT2B

Immunogen:
Recombinant protein corresponding to human PCAF (a.a. 782-832).

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_003875

Target Species:
Human

Applications:
ChIP Assay, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
Q92831

Host Species:
Rabbit

Purification Method:
__

Regulatory Status:
RUO

Primary or Secondary:
Primary

Includes:
This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.

Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Img

MilliporeSigma™

17-105-33MI

--


Antigen:
CHD1

Classification:
Polyclonal

Formulation:
Anti-CHD1 (Rabbit Polyclonal). One vial containing 7.0μg purified rabbit polyclonal in 50μL buffer of Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide, before the addition of 30% glycerol (0.14mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, PSMC6. One vial containing 75μL of 5μM of each primer specific for human proteasome 26S subunit 6 (chr14:53174161+53174274 , hg19 build). Primer location selection based on ENCODE browser data at UCSC, see also reference 1. FOR: TCC GGC CCT GAG CTT GT; REV: CCT CCT CTA CTT CTT TTT CAT TTT CAC

Gene Symbols:
Chd1, Chd-1

Immunogen:
Recombinant protein corresponding to human CHD1 (a.a. 1660-1710).

Quantity:
10 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_001261

Target Species:
Human

Applications:
ChIP Assay, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
O14646

Host Species:
Rabbit

Purification Method:
__

Regulatory Status:
RUO

Primary or Secondary:
Primary

Includes:
This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.

Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

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Invitrogen

17-108-382

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Antigen:
VISTA

Classification:
Monoclonal

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Symbols:
VSIR

Immunogen:
__

Quantity:
100 μg

Research Discipline:
__

Gene ID (Entrez):
74048

Target Species:
Mouse

Applications:
Flow Cytometry

Conjugate:
APC

Gene Accession No.:
Q9D659

Host Species:
Rat

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Primary or Secondary:
Primary

Includes:
__

Content And Storage:
4° C, store in dark, DO NOT FREEZE!