5539389

Cytokeratin 18 Antibody (KRT18/835), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Cytokeratin 18

Concentration

0.2mg/mL

Applications

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias

Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18

Gene Symbols

KRT18

Isotype

IgG1

Purification Method

Protein A or G purified

Test Specificity

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Clone

KRT18/835

Dilution

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative)

Gene Accession No.

P05783

Gene ID (Entrez)

3875

Immunogen

Recombinant full-length human KRT18 protein

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

45 kDa

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Description

  • Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence Cytokeratin 18 Monoclonal specifically detects Cytokeratin 18 in Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative) samples
  • It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Protein Array.

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Img

Fischer Scientific

5539389

Mouse Monoclonal Antibody...


Antigen:
Cytokeratin 18

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18

Gene Symbols:
KRT18

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Clone:
KRT18/835

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative)

Gene Accession No.:
P05783

Gene ID (Entrez):
3875

Immunogen:
Recombinant full-length human KRT18 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
45 kDa

Img

Fischer Scientific

5539390

Mouse Monoclonal Antibody...


Antigen:
Cytokeratin 18

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18

Gene Symbols:
KRT18

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Clone:
KRT18/836

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 2 - 4 ug/ml, Immunofluorescence 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Canine, Feline, Rat (Negative)

Gene Accession No.:
__

Gene ID (Entrez):
3875

Immunogen:
Recombinant human KRT18 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
45 kDa

Img

Fischer Scientific

5539390

Mouse Monoclonal Antibody...


Antigen:
Cytokeratin 18

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18

Gene Symbols:
KRT18

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Clone:
KRT18/836

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 2 - 4 ug/ml, Immunofluorescence 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Canine, Feline, Rat (Negative)

Gene Accession No.:
__

Gene ID (Entrez):
3875

Immunogen:
Recombinant human KRT18 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
45 kDa

Img

Fischer Scientific

5539392

Mouse Monoclonal Antibody...


Antigen:
Cytokeratin 19

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Neuroscience, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
CK19, CK-19, cytokeratin 19, Cytokeratin-19,40-kDa keratin intermediate filament, K19cytokeratin-19, K1CS, keratin 19, keratin, type I cytoskeletal 19, keratin, type I, 40-kd, keratin-19, MGC15366

Gene Symbols:
KRT19

Isotype:
IgG1, IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

Clone:
KRT19/799 + KRT19/800

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
P08727

Gene ID (Entrez):
3880

Immunogen:
Recombinant full-length human KRT19 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
40 kDa