5540098

Pax7 Antibody (SPM613), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 1 publication

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Pax7

Concentration

0.2mg/mL

Applications

Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Apoptosis, Mesenchymal Stem Cell Markers, Stem Cell Markers

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias

FLJ37460, HuP1, paired box 7, paired box gene 7, paired box homeotic gene 7, paired box protein Pax-7, paired domain gene 7, PAX7 transcriptional factor, PAX7B, RMS2

Gene Symbols

PAX7

Isotype

IgG1 κ

Purification Method

Protein A or G purified

Test Specificity

The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

Clone

SPM613

Dilution

Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Accession No.

P23759

Gene ID (Entrez)

5081

Immunogen

Recombinant fragment (aa300-600) of human PAX7 protein

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

57 kDa

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Description

  • Ensure accurate, reproducible results in Immunohistochemistry (Paraffin) Pax7 Monoclonal specifically detects Pax7 in Human, Mouse samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

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Mouse Monoclonal Antibody has been ...


Antigen:
Pax7

Concentration:
0.2mg/mL

Applications:
Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Apoptosis, Mesenchymal Stem Cell Markers, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
FLJ37460, HuP1, paired box 7, paired box gene 7, paired box homeotic gene 7, paired box protein Pax-7, paired domain gene 7, PAX7 transcriptional factor, PAX7B, RMS2

Gene Symbols:
PAX7

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

Clone:
SPM613

Dilution:
Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P23759

Gene ID (Entrez):
5081

Immunogen:
Recombinant fragment (aa300-600) of human PAX7 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
57 kDa

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5540105

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Antigen:
PCNA

Concentration:
0.2mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Autophagy, Base Excision Repair, Cell Cycle and Replication, Cellular Markers, Core ESC Like Genes, DNA Polymerases, DNA Repair, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbols:
PCNA

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Clone:
PCNA/694

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P12004

Gene ID (Entrez):
5111

Immunogen:
Recombinant full length human PCNA protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
36 kDa

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5540128

Mouse Monoclonal Antibody...


Antigen:
UCH-L1/PGP9.5

Concentration:
0.2 mg/mL

Applications:
Western Blot, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Alzheimers Research, Cellular Markers, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Neurotransmission

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
EC 3.4.19.12, EC 6.-, Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, PGP9.5Uch-L1, PGP95, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), ubiquitin carboxyl-terminal hydrolase isozyme L1, ubiquitin C-terminal hydrolase, Ubiquitin thioesterase L1, UCHL1, UCH-L1

Gene Symbols:
UCHL1

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.

Clone:
UCHL1/841

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
__

Gene ID (Entrez):
7345

Immunogen:
Recombinant human UCHL1 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
__

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5540137

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Antigen:
Phospho-Tyrosine

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
__

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
2 amino 3(4 hydroxyphenyl) propanoic acid, 4 hydroxyphenylalanine, phosphotyrosine, pTyrosine, Tyrosine

Gene Symbols:
__

Isotype:
IgG2b

Purification Method:
Protein A or G purified

Test Specificity:
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Clone:
PY793

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 1 - 2 ug/ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, All species

Gene Accession No.:
__

Gene ID (Entrez):
__

Immunogen:
Phosphotyrosine conjugated to BSA

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
__