CD45 Mouse, Clone: 2B11 + PD7/26, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 2 publications
Manufacturer: Fischer Scientific
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Antigen
CD45
Dilution
Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Canine
Gene Accession No.
P08575
Gene ID (Entrez)
5788
Immunogen
Isolated neoplastic cells from T cell lymphoma (2B11); and human peripheral blood lymphocytes maintained in T cell growth factor (PD7/26)
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Clone
2B11 + PD7/26
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, MAP Kinase Signaling, Mast Cell Markers, Mesenchymal Stem Cell Markers, Microglia Markers, Modulation of DNA Pools, Myeloid Cell Markers, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Formulation
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols
PTPRC
Isotype
IgG
Purification Method
Protein A purified
Test Specificity
Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
Related Products
Description
- CD45 Monoclonal specifically detects CD45 in Human, Canine samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.
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Antigen: CD45
Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Canine
Gene Accession No.: P08575
Gene ID (Entrez): 5788
Immunogen: Isolated neoplastic cells from T cell lymphoma (2B11); and human peripheral blood lymphocytes maintained in T cell growth factor (PD7/26)
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: 2B11 + PD7/26
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, MAP Kinase Signaling, Mast Cell Markers, Mesenchymal Stem Cell Markers, Microglia Markers, Modulation of DNA Pools, Myeloid Cell Markers, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols: PTPRC
Isotype: IgG
Purification Method: Protein A purified
Test Specificity: Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
Antigen: Bcl-xL
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine
Gene Accession No.: Q07817
Gene ID (Entrez): 598
Immunogen: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein (BX006 & 2H12)
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: BX006 + 2H12
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Cancer, Tumor Suppressors
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: Apoptosis regulator Bcl-X, bcl2-L-1, BCL2-like 1, bcl-2-like protein 1, BCLXBCL2LBcl-X, bcl-xL, BCLXL, BCL-XL/S, bcl-xS, BCLXS, DKFZp781P2092
Gene Symbols: BCL2L1
Isotype: IgG
Purification Method: Protein A purified
Test Specificity: Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.
Antigen: CD98
Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1.0ug/ml, Immunoprecipitation 1-2ug/500ug protein, Immunohistochemistry-Frozen 0.5-1ug/ml, SDS-Page
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Accession No.: P08195
Gene ID (Entrez): 6520
Immunogen: Molt 13 T cell line
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: UM7F8
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Biology, Cellular Markers, Immunology, Lipid and Metabolism, Plasma Membrane Markers, Signal Transduction
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: 4F2, 4F2hc, 4T2HC, antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC, CD98, CD98 antigen, CD98 heavy chain, CD98HC, heavy chain, Lymphocyte activation antigen 4F2 large subunit, MDU1antigen defined by monoclonal 4F2, heavy chain, monoclonal 44D7, NACAE4F2 cell-surface antigen heavy chain, solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen
Gene Symbols: SLC3A2
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: CD98 exits as a heterodimer containing a disulphide-linked glycosylated heavy chain and a non-glycosylated light chain. It is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through disulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.
