7105585

CDC2/CDK1 Mouse, Clone: POH-1; same as cdc2.1, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 1 publication

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CDC2/CDK1

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1-2ug/ml, SDS-Page

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Bovine, Mustelid, Primate, Drosophila (Negative), Mouse (Negative), Rat (Negative), Xenopus (Negative)

Gene Accession No.

P06493

Gene ID (Entrez)

983

Immunogen

Purified recombinant human p34cdc2 fusion protein

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

34 kDa

Clone

POH-1; same as cdc2.1

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Breast Cancer, Cancer, Cell Cycle and Replication, Core ESC Like Genes, Mitotic Regulators, Stem Cell Markers

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

CDC28A, CDC2MGC111195, cell cycle controller CDC2, Cell division control protein 2 homolog, Cell division protein kinase 1, cyclin-dependent kinase 1, DKFZp686L20222, EC 2.7.11.22, EC 2.7.11.23, G1 to S and G2 to M, p34 protein kinase, P34CDC2

Gene Symbols

CDK1

Isotype

IgG2a κ

Purification Method

Protein A purified

Test Specificity

Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. cdk1/ p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated cdk1/ p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

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Description

  • CDC2/CDK1 Monoclonal specifically detects CDC2/CDK1 in Human, Bovine, Mink, Mustelid, Monkey, Drosophila (Negative), Mouse (Negative), Rat (Negative), Xenopus (Negative) samples
  • It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

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7105585

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Antigen:
CDC2/CDK1

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1-2ug/ml, SDS-Page

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Bovine, Mustelid, Primate, Drosophila (Negative), Mouse (Negative), Rat (Negative), Xenopus (Negative)

Gene Accession No.:
P06493

Gene ID (Entrez):
983

Immunogen:
Purified recombinant human p34cdc2 fusion protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
34 kDa

Clone:
POH-1; same as cdc2.1

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Breast Cancer, Cancer, Cell Cycle and Replication, Core ESC Like Genes, Mitotic Regulators, Stem Cell Markers

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
CDC28A, CDC2MGC111195, cell cycle controller CDC2, Cell division control protein 2 homolog, Cell division protein kinase 1, cyclin-dependent kinase 1, DKFZp686L20222, EC 2.7.11.22, EC 2.7.11.23, G1 to S and G2 to M, p34 protein kinase, P34CDC2

Gene Symbols:
CDK1

Isotype:
IgG2a κ

Purification Method:
Protein A purified

Test Specificity:
Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. cdk1/ p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated cdk1/ p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

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7105591

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Antigen:
Cytokeratin 8/18

Dilution:
Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P05787

Gene ID (Entrez):
3856

Immunogen:
Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10)

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
__

Clone:
K8.8 + DC10

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cytoskeleton Markers

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8

Gene Symbols:
KRT8

Isotype:
IgG

Purification Method:
Protein A purified

Test Specificity:
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.

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7105592

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Antigen:
Chorionic Gonadotropin beta Chain (HCG beta)

Dilution:
Western Blot 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P01233

Gene ID (Entrez):
1082

Immunogen:
Recombinant hCG beta protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
22 kDa

Clone:
SPM105

Applications:
Western Blot, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
CGB3choriogonadotropin subunit beta, CGB5, CGB7, CGB8, CG-beta, Chorionic gonadotrophin chain beta, chorionic gonadotropin beta 3 subunit, chorionic gonadotropin beta chain, chorionic gonadotropin beta subunit, chorionic gonadotropin, beta polypeptide, hCGB

Gene Symbols:
CGB

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
This MAb reacts with a protein of 22kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit.

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7105595

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Antigen:
GFAP

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Porcine, Bovine, Chicken, Rabbit

Gene Accession No.:
P14136

Gene ID (Entrez):
2670

Immunogen:
GFAP isolated from pig spinal cord

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
50 kDa

Clone:
SPM248

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Astrocyte Markers, Cancer, Cellular Markers, Cytoskeleton Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
FLJ45472, GFAP astrocytes, glial fibrillary acidic protein

Gene Symbols:
GFAP

Isotype:
IgG1

Purification Method:
Protein G purified

Test Specificity:
This MAb recognizes a protein of ∼50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.