CD8a Monoclonal Antibody (YCATE55.9), PE, eBioscience™, Invitrogen™

Description

• Description: This YCATE55.9 monoclonal antibody reacts with canine CD8 alpha
• This cell surface receptor is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha)
• In dogs, CD8 alpha is expressed on thymocytes, a subset of mature T cells, NK cells, and intestinal intraepithelial lymphocytes (IELs)
• CD8 binds to MHC class I and plays a role in thymocyte development and mature T cell activation
• This monoclonal antibody has been reported to inhibit cytotoxic T cell generation and function in vitro
• In addition, this antibody has been reported to be immunosuppressive in vivo
• Applications Reported: This YCATE55.9 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This YCATE55.9 antibody has been pre-diluted and tested by flow cytometric analysis of normal canine peripheral blood cells
• This may be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation
• CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules
• CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I
• CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha
• Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation
• Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells
• Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.