7182756
CD40/TNFRSF5, Rabbit anti-Human, Clone: A54 scFv, Novus Biologicals™
Rabbit Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD40/TNFRSF5
Concentration
1 mg/mL
Applications
ELISA, Functional Assay, Blocking Assay
Conjugate
Unconjugated
Host Species
Rabbit
Research Discipline
Adaptive Immunity, Apoptosis, Asthma, B Cell Development and Differentiation Markers, Cancer, Hematopoietic Stem Cell Markers, Immunology, Signal Transduction, Stem Cell Markers
Formulation
PBS
Gene ID (Entrez)
958
Immunogen
A54 human anti-CD40/TNFRSF5 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fcgamma fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.
Primary or Secondary
Primary
Content And Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone
A54 scFv
Dilution
ELISA, Functional, Block/Neutralize
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40 antigen, CD40 molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, nerve growth factor receptor-related B-lymphocyte activation molecule, p50, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5
Gene Symbols
CD40
Isotype
IgG λ
Purification Method
Protein A purified
Test Specificity
A54 binds specifically to the D1 domain of CD40/TNFRSF5 (and can only bind to the intact i.e. non-truncated version of CD40/TNFRSF5 and when an AD2 peptide tag has not been added) and can recognize CD40/TNFRSF5 on a B cell line (BJAB). This scFv binds to CD40/TNFRSF5 (immobilized by amine coupling) with a KD within the range of 40nM. CD40/TNFRSF5 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40/TNFRSF5 binds to the membrane protein CD154 of the TNF superfamily and this interaction is required for the seconfary immune responses and germinal centre formation.
Related Products
Description
- CD40/TNFRSF5 Monoclonal specifically detects CD40/TNFRSF5 in Human samples
- It is validated for ELISA, Immunocytochemistry/Immunofluorescence, Functional, Block/Neutralize.
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Antigen: CD40/TNFRSF5
Concentration: 1 mg/mL
Applications: ELISA, Functional Assay, Blocking Assay
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Adaptive Immunity, Apoptosis, Asthma, B Cell Development and Differentiation Markers, Cancer, Hematopoietic Stem Cell Markers, Immunology, Signal Transduction, Stem Cell Markers
Formulation: PBS
Gene ID (Entrez): 958
Immunogen: A54 human anti-CD40/TNFRSF5 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fcgamma fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone: A54 scFv
Dilution: ELISA, Functional, Block/Neutralize
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40 antigen, CD40 molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, nerve growth factor receptor-related B-lymphocyte activation molecule, p50, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5
Gene Symbols: CD40
Isotype: IgG λ
Purification Method: Protein A purified
Test Specificity: A54 binds specifically to the D1 domain of CD40/TNFRSF5 (and can only bind to the intact i.e. non-truncated version of CD40/TNFRSF5 and when an AD2 peptide tag has not been added) and can recognize CD40/TNFRSF5 on a B cell line (BJAB). This scFv binds to CD40/TNFRSF5 (immobilized by amine coupling) with a KD within the range of 40nM. CD40/TNFRSF5 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40/TNFRSF5 binds to the membrane protein CD154 of the TNF superfamily and this interaction is required for the seconfary immune responses and germinal centre formation.
Antigen:
CD40/TNFRSF5
Concentration:
1 mg/mL
Applications:
ELISA, Functional Assay, Blocking Assay
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Adaptive Immunity, Apoptosis, Asthma, B Cell Development and Differentiation Markers, Cancer, Hematopoietic Stem Cell Markers, Immunology, Signal Transduction, Stem Cell Markers
Formulation:
PBS
Gene ID (Entrez):
958
Immunogen:
A54 human anti-CD40/TNFRSF5 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fcgamma fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone:
A54 scFv
Dilution:
ELISA, Functional, Block/Neutralize
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40 antigen, CD40 molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, nerve growth factor receptor-related B-lymphocyte activation molecule, p50, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5
Gene Symbols:
CD40
Isotype:
IgG λ
Purification Method:
Protein A purified
Test Specificity:
A54 binds specifically to the D1 domain of CD40/TNFRSF5 (and can only bind to the intact i.e. non-truncated version of CD40/TNFRSF5 and when an AD2 peptide tag has not been added) and can recognize CD40/TNFRSF5 on a B cell line (BJAB). This scFv binds to CD40/TNFRSF5 (immobilized by amine coupling) with a KD within the range of 40nM. CD40/TNFRSF5 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40/TNFRSF5 binds to the membrane protein CD154 of the TNF superfamily and this interaction is required for the seconfary immune responses and germinal centre formation.
Antigen: EDAR
Concentration: 1 mg/mL
Applications: Western Blot, ELISA, Blocking Assay
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Apoptosis
Formulation: PBS
Gene ID (Entrez): 10913
Immunogen: EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone: EDAR12
Dilution: Western Blot, ELISA, Surface Plasmon Resonance, Block/Neutralize
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Canine, Chicken
Gene Alias: Anhidrotic ectodysplasin receptor 1, DLED3, Ectodermal dysplasia receptor, ectodysplasin 1, anhidrotic receptor, ectodysplasin A receptor, Ectodysplasin-A receptor, ED1R, ED5, EDA1R, EDA3EDA-A1 receptor, EDA-A1R, Edar, FLJ94390, HRM1, mouse, homolog of, tumor necrosis factor receptor superfamily member EDAR
Gene Symbols: EDAR
Isotype: IgG κ
Purification Method: Protein A purified
Test Specificity: EDAR12 recognises and binds to CRD1 of the extracellular domain of mouse EDAR. EDAR3 cross-reacts with EDAR derived from human, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.
Antigen:
EDAR
Concentration:
1 mg/mL
Applications:
Western Blot, ELISA, Blocking Assay
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Apoptosis
Formulation:
PBS
Gene ID (Entrez):
10913
Immunogen:
EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone:
EDAR12
Dilution:
Western Blot, ELISA, Surface Plasmon Resonance, Block/Neutralize
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Canine, Chicken
Gene Alias:
Anhidrotic ectodysplasin receptor 1, DLED3, Ectodermal dysplasia receptor, ectodysplasin 1, anhidrotic receptor, ectodysplasin A receptor, Ectodysplasin-A receptor, ED1R, ED5, EDA1R, EDA3EDA-A1 receptor, EDA-A1R, Edar, FLJ94390, HRM1, mouse, homolog of, tumor necrosis factor receptor superfamily member EDAR
Gene Symbols:
EDAR
Isotype:
IgG κ
Purification Method:
Protein A purified
Test Specificity:
EDAR12 recognises and binds to CRD1 of the extracellular domain of mouse EDAR. EDAR3 cross-reacts with EDAR derived from human, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.
Antigen: Lymphotoxin-alpha/TNF-beta
Concentration: 1 mg/mL
Applications: Flow Cytometry, ELISA, Neutralization
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Apoptosis, Asthma, Biologically Active Proteins, Cytokine Research, Immunology, Innate Immunity
Formulation: PBS
Gene ID (Entrez): 4049
Immunogen: 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone: 3F12.2D3
Dilution: Flow Cytometry, ELISA, Surface Plasmon Resonance, Neutralization
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: LT, LT-alpha, lymphotoxin alpha (TNF superfamily, member 1), TNFBlymphotoxin-alpha, TNFSF1TNF-beta, tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1
Gene Symbols: LTA
Isotype: IgG2b κ
Purification Method: Protein A purified
Test Specificity: 3F12 binds to human LTalpha3 and LT-alpha-beta (with a KD of ∽0.3 nM, determined by BIACORE; ∽ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTalpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTalpha3), or is expressed on the cell surface in complex with LTbeta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTalpha3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
Antigen:
Lymphotoxin-alpha/TNF-beta
Concentration:
1 mg/mL
Applications:
Flow Cytometry, ELISA, Neutralization
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Adaptive Immunity, Apoptosis, Asthma, Biologically Active Proteins, Cytokine Research, Immunology, Innate Immunity
Formulation:
PBS
Gene ID (Entrez):
4049
Immunogen:
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone:
3F12.2D3
Dilution:
Flow Cytometry, ELISA, Surface Plasmon Resonance, Neutralization
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
LT, LT-alpha, lymphotoxin alpha (TNF superfamily, member 1), TNFBlymphotoxin-alpha, TNFSF1TNF-beta, tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1
Gene Symbols:
LTA
Isotype:
IgG2b κ
Purification Method:
Protein A purified
Test Specificity:
3F12 binds to human LTalpha3 and LT-alpha-beta (with a KD of ∽0.3 nM, determined by BIACORE; ∽ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTalpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTalpha3), or is expressed on the cell surface in complex with LTbeta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTalpha3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
Antigen: Glycophorin A
Concentration: 1 mg/mL
Applications: ELISA, Immunoblot, Inhibition Assays
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Cancer, Signal Transduction
Formulation: PBS
Gene ID (Entrez): 2993
Immunogen: M2A1 was prepared by immunzing BALB/c mice with human red blood cells of the M phenotype, and was screened by testing for anti-M activity.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone: M2A1
Dilution: ELISA, Immunoblotting, Functional (Inhibition)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CD235a antigen, glycophorin A (includes MN blood group), glycophorin A (MNS blood group), glycophorin Erik, glycophorin MiI, glycophorin MiIII, glycophorin MiV, glycophorin MiX, glycophorin SAT, glycophorin Sta type C, glycophorin-A, GPA, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), Mi.V glycoprotein (24 AA), MN sialoglycoprotein, MNS, PAS-2, recombinant glycophorin A-B Miltenberger-DR
Gene Symbols: GYPA
Isotype: IgG κ
Purification Method: Protein A purified
Test Specificity: M2A1 binds specifically to the M antigen on human erythrocytes, and shows no reactivity to N antigen. The epitope for this antibody includes the amino-terminal serine residue and sialic acid residues of glycophorin A. The reactivity of M2A1 for M antigen is pH- and salt-dependent (optimum ∽ pH8-9). Blood group antigen M is located on the major sialoglycoprotein (glycophorin A) of red blood cells.
Antigen:
Glycophorin A
Concentration:
1 mg/mL
Applications:
ELISA, Immunoblot, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Cancer, Signal Transduction
Formulation:
PBS
Gene ID (Entrez):
2993
Immunogen:
M2A1 was prepared by immunzing BALB/c mice with human red blood cells of the M phenotype, and was screened by testing for anti-M activity.
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone:
M2A1
Dilution:
ELISA, Immunoblotting, Functional (Inhibition)
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CD235a antigen, glycophorin A (includes MN blood group), glycophorin A (MNS blood group), glycophorin Erik, glycophorin MiI, glycophorin MiIII, glycophorin MiV, glycophorin MiX, glycophorin SAT, glycophorin Sta type C, glycophorin-A, GPA, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), Mi.V glycoprotein (24 AA), MN sialoglycoprotein, MNS, PAS-2, recombinant glycophorin A-B Miltenberger-DR
Gene Symbols:
GYPA
Isotype:
IgG κ
Purification Method:
Protein A purified
Test Specificity:
M2A1 binds specifically to the M antigen on human erythrocytes, and shows no reactivity to N antigen. The epitope for this antibody includes the amino-terminal serine residue and sialic acid residues of glycophorin A. The reactivity of M2A1 for M antigen is pH- and salt-dependent (optimum ∽ pH8-9). Blood group antigen M is located on the major sialoglycoprotein (glycophorin A) of red blood cells.