Fas/TNFRSF6/CD95, Human anti-Human, Clone: R-125224, Novus Biologicals™
Human Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Fas/TNFRSF6/CD95
Concentration
1 mg/mL
Applications
Flow Cytometry, ELISA, Functional Assay
Conjugate
Unconjugated
Host Species
Human
Target Species
Human
Gene Alias
Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6
Gene Symbols
FAS
Isotype
IgG1 κ
Purification Method
Protein A purified
Test Specificity
R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.
Clone
R-125224
Dilution
Flow Cytometry, ELISA, Functional
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Formulation
PBS
Gene ID (Entrez)
355
Immunogen
R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.
Primary or Secondary
Primary
Content And Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Related Products
Description
- Fas/TNFRSF6/CD95 Monoclonal specifically detects Fas/TNFRSF6/CD95 in Human samples
- It is validated for Flow Cytometry, ELISA, Functional.
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Antigen: Fas/TNFRSF6/CD95
Concentration: 1 mg/mL
Applications: Flow Cytometry, ELISA, Functional Assay
Conjugate: Unconjugated
Host Species: Human
Target Species: Human
Gene Alias: Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6
Gene Symbols: FAS
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.
Clone: R-125224
Dilution: Flow Cytometry, ELISA, Functional
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS
Gene ID (Entrez): 355
Immunogen: R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Antigen: TIGIT
Concentration: 1 mg/mL
Applications: Flow Cytometry, Blocking Assay
Conjugate: Unconjugated
Host Species: Armenian Hamster
Target Species: Mouse
Gene Alias: T cell immunoreceptor with Ig and ITIM domains, TIGIT, VSIG9, VSTM3, WUCAM
Gene Symbols: TIGIT
Isotype: IgG κ
Purification Method: Protein A purified
Test Specificity: 4D4 specifically recognises TIGIT.
Clone: 4D4
Dilution: Flow Cytometry, Block/Neutralize
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS
Gene ID (Entrez): 201633
Immunogen: Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Antigen: TIM-1/KIM-1/HAVCR
Concentration: 1 mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Mouse
Gene Alias: HAVCR-1, HAVCRT cell immunoglobin domain and mucin domain protein 1, hepatitis A virus cellular receptor 1, Kidney injury molecule 1, KIM1, KIM-1, T-cell immunoglobulin and mucin domain-containing protein 1, TIM-1TIM, TIM1TIMD-1, TIMD1T-cell membrane protein 1
Gene Symbols: HAVCR1
Isotype: IgG κ
Purification Method: Protein A purified
Test Specificity: This antibody is specific for murine TIM-1/KIM-1/HAVCR, a member of the T cell immunoglobulin domain, mucin-like domain (TIM) gene family. TIM-1/KIM-1/HAVCR has been found on activated CD4+ T cells, mast cells, and a subset of B cells. TIM-1/KIM-1/HAVCR is involved in renal injury, tissue homeostasis, and T cell costimulation, and promotes the differentiation of Th2 cells and the production of IL-4. TIM-1/KIM-1/HAVCR can bind several ligands, including Hepatitis A virus, phosphatidylserine, TIM4, IgA, as well as itself.
Clone: 3B3
Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS
Gene ID (Entrez): 26762
Immunogen: This antibody was raised by immunising female Lewis strain rats (Harlan Sprague-Dawley) subcutaneously with mouse TIM-1/KIM-1/HAVCR-Ig in complete Freund's adjuvant (CFA), followed by multiple 'boosting' with mouse TIM-1/KIM-1/HAVCR-Ig in PBS.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.