7182761
Lymphotoxin-alpha/TNF-beta, Mouse anti-Human, Clone: 3F12.2D3, Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Lymphotoxin-alpha/TNF-beta
Concentration
1 mg/mL
Applications
Flow Cytometry, ELISA, Neutralization
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Adaptive Immunity, Apoptosis, Asthma, Biologically Active Proteins, Cytokine Research, Immunology, Innate Immunity
Formulation
PBS
Gene ID (Entrez)
4049
Immunogen
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Primary or Secondary
Primary
Content And Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone
3F12.2D3
Dilution
Flow Cytometry, ELISA, Surface Plasmon Resonance, Neutralization
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
LT, LT-alpha, lymphotoxin alpha (TNF superfamily, member 1), TNFBlymphotoxin-alpha, TNFSF1TNF-beta, tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1
Gene Symbols
LTA
Isotype
IgG2b κ
Purification Method
Protein A purified
Test Specificity
3F12 binds to human LTalpha3 and LT-alpha-beta (with a KD of ∽0.3 nM, determined by BIACORE; ∽ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTalpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTalpha3), or is expressed on the cell surface in complex with LTbeta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTalpha3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
Related Products
Description
- Lymphotoxin-alpha/TNF-beta Monoclonal specifically detects Lymphotoxin-alpha/TNF-beta in Human samples
- It is validated for Flow Cytometry, ELISA, Surface Plasmon Resonance, Neutralization.
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Antigen: Lymphotoxin-alpha/TNF-beta
Concentration: 1 mg/mL
Applications: Flow Cytometry, ELISA, Neutralization
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Apoptosis, Asthma, Biologically Active Proteins, Cytokine Research, Immunology, Innate Immunity
Formulation: PBS
Gene ID (Entrez): 4049
Immunogen: 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone: 3F12.2D3
Dilution: Flow Cytometry, ELISA, Surface Plasmon Resonance, Neutralization
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: LT, LT-alpha, lymphotoxin alpha (TNF superfamily, member 1), TNFBlymphotoxin-alpha, TNFSF1TNF-beta, tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1
Gene Symbols: LTA
Isotype: IgG2b κ
Purification Method: Protein A purified
Test Specificity: 3F12 binds to human LTalpha3 and LT-alpha-beta (with a KD of ∽0.3 nM, determined by BIACORE; ∽ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTalpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTalpha3), or is expressed on the cell surface in complex with LTbeta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTalpha3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
Antigen:
Lymphotoxin-alpha/TNF-beta
Concentration:
1 mg/mL
Applications:
Flow Cytometry, ELISA, Neutralization
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Adaptive Immunity, Apoptosis, Asthma, Biologically Active Proteins, Cytokine Research, Immunology, Innate Immunity
Formulation:
PBS
Gene ID (Entrez):
4049
Immunogen:
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone:
3F12.2D3
Dilution:
Flow Cytometry, ELISA, Surface Plasmon Resonance, Neutralization
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
LT, LT-alpha, lymphotoxin alpha (TNF superfamily, member 1), TNFBlymphotoxin-alpha, TNFSF1TNF-beta, tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1
Gene Symbols:
LTA
Isotype:
IgG2b κ
Purification Method:
Protein A purified
Test Specificity:
3F12 binds to human LTalpha3 and LT-alpha-beta (with a KD of ∽0.3 nM, determined by BIACORE; ∽ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTalpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTalpha3), or is expressed on the cell surface in complex with LTbeta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTalpha3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
Antigen: Glycophorin A
Concentration: 1 mg/mL
Applications: ELISA, Immunoblot, Inhibition Assays
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Cancer, Signal Transduction
Formulation: PBS
Gene ID (Entrez): 2993
Immunogen: M2A1 was prepared by immunzing BALB/c mice with human red blood cells of the M phenotype, and was screened by testing for anti-M activity.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone: M2A1
Dilution: ELISA, Immunoblotting, Functional (Inhibition)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CD235a antigen, glycophorin A (includes MN blood group), glycophorin A (MNS blood group), glycophorin Erik, glycophorin MiI, glycophorin MiIII, glycophorin MiV, glycophorin MiX, glycophorin SAT, glycophorin Sta type C, glycophorin-A, GPA, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), Mi.V glycoprotein (24 AA), MN sialoglycoprotein, MNS, PAS-2, recombinant glycophorin A-B Miltenberger-DR
Gene Symbols: GYPA
Isotype: IgG κ
Purification Method: Protein A purified
Test Specificity: M2A1 binds specifically to the M antigen on human erythrocytes, and shows no reactivity to N antigen. The epitope for this antibody includes the amino-terminal serine residue and sialic acid residues of glycophorin A. The reactivity of M2A1 for M antigen is pH- and salt-dependent (optimum ∽ pH8-9). Blood group antigen M is located on the major sialoglycoprotein (glycophorin A) of red blood cells.
Antigen:
Glycophorin A
Concentration:
1 mg/mL
Applications:
ELISA, Immunoblot, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Cancer, Signal Transduction
Formulation:
PBS
Gene ID (Entrez):
2993
Immunogen:
M2A1 was prepared by immunzing BALB/c mice with human red blood cells of the M phenotype, and was screened by testing for anti-M activity.
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone:
M2A1
Dilution:
ELISA, Immunoblotting, Functional (Inhibition)
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CD235a antigen, glycophorin A (includes MN blood group), glycophorin A (MNS blood group), glycophorin Erik, glycophorin MiI, glycophorin MiIII, glycophorin MiV, glycophorin MiX, glycophorin SAT, glycophorin Sta type C, glycophorin-A, GPA, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), Mi.V glycoprotein (24 AA), MN sialoglycoprotein, MNS, PAS-2, recombinant glycophorin A-B Miltenberger-DR
Gene Symbols:
GYPA
Isotype:
IgG κ
Purification Method:
Protein A purified
Test Specificity:
M2A1 binds specifically to the M antigen on human erythrocytes, and shows no reactivity to N antigen. The epitope for this antibody includes the amino-terminal serine residue and sialic acid residues of glycophorin A. The reactivity of M2A1 for M antigen is pH- and salt-dependent (optimum ∽ pH8-9). Blood group antigen M is located on the major sialoglycoprotein (glycophorin A) of red blood cells.
Antigen: Glycophorin A
Concentration: 1 mg/mL
Applications: ELISA, Immunoblot, Inhibition Assays
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Cancer, Signal Transduction
Formulation: PBS
Gene ID (Entrez): 2993
Immunogen: M2A1 was prepared by immunzing BALB/c mice with human red blood cells of the M phenotype, and was screened by testing for anti-M activity.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone: M2A1
Dilution: ELISA, Immunoblotting, Functional (Inhibition)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CD235a antigen, glycophorin A (includes MN blood group), glycophorin A (MNS blood group), glycophorin Erik, glycophorin MiI, glycophorin MiIII, glycophorin MiV, glycophorin MiX, glycophorin SAT, glycophorin Sta type C, glycophorin-A, GPA, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), Mi.V glycoprotein (24 AA), MN sialoglycoprotein, MNS, PAS-2, recombinant glycophorin A-B Miltenberger-DR
Gene Symbols: GYPA
Isotype: IgG κ
Purification Method: Protein A purified
Test Specificity: M2A1 binds specifically to the M antigen on human erythrocytes, and shows no reactivity to N antigen. The epitope for this antibody includes the amino-terminal serine residue and sialic acid residues of glycophorin A. The reactivity of M2A1 for M antigen is pH- and salt-dependent (optimum ∽ pH8-9). Blood group antigen M is located on the major sialoglycoprotein (glycophorin A) of red blood cells.
Antigen:
Glycophorin A
Concentration:
1 mg/mL
Applications:
ELISA, Immunoblot, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Cancer, Signal Transduction
Formulation:
PBS
Gene ID (Entrez):
2993
Immunogen:
M2A1 was prepared by immunzing BALB/c mice with human red blood cells of the M phenotype, and was screened by testing for anti-M activity.
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone:
M2A1
Dilution:
ELISA, Immunoblotting, Functional (Inhibition)
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CD235a antigen, glycophorin A (includes MN blood group), glycophorin A (MNS blood group), glycophorin Erik, glycophorin MiI, glycophorin MiIII, glycophorin MiV, glycophorin MiX, glycophorin SAT, glycophorin Sta type C, glycophorin-A, GPA, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), Mi.V glycoprotein (24 AA), MN sialoglycoprotein, MNS, PAS-2, recombinant glycophorin A-B Miltenberger-DR
Gene Symbols:
GYPA
Isotype:
IgG κ
Purification Method:
Protein A purified
Test Specificity:
M2A1 binds specifically to the M antigen on human erythrocytes, and shows no reactivity to N antigen. The epitope for this antibody includes the amino-terminal serine residue and sialic acid residues of glycophorin A. The reactivity of M2A1 for M antigen is pH- and salt-dependent (optimum ∽ pH8-9). Blood group antigen M is located on the major sialoglycoprotein (glycophorin A) of red blood cells.
Antigen: CD98
Concentration: 1 mg/mL
Applications: ELISA, Immunofluorescence, Blocking Assay
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Biologically Active Proteins, Cell Biology, Cellular Markers, Immunology, Lipid and Metabolism, Plasma Membrane Markers, Signal Transduction
Formulation: PBS
Gene ID (Entrez): 6520
Immunogen: HBJ127 was obtained from hybridomas generated by the fusion of mouse myeloma cells and spleen cells from mice which had been immunized with T24 hyman bladder cancer cells.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone: HBJ127
Dilution: ELISA, Immunocytochemistry/Immunofluorescence, Block/Neutralize
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: 4F2, 4F2hc, 4T2HC, antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC, CD98, CD98 antigen, CD98 heavy chain, CD98HC, heavy chain, Lymphocyte activation antigen 4F2 large subunit, MDU1antigen defined by monoclonal 4F2, heavy chain, monoclonal 44D7, NACAE4F2 cell-surface antigen heavy chain, solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen
Gene Symbols: SLC3A2
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: HBJ127 binds to the CD98 heavy chain at an epitope consisting of residues AFS (aa 442-444) (Itoh et al, 2007). The antibody has not been shown to cross-react with other species. CD98 is expressed at high levels on monocytes and at very low level on peripheral blood T and B lymphocytes, splenocytes, NK cells and granulocytes. CD98 is a disulfide-linked and glycosylated type II integral membrane protein. This protein has roles in normal and neoplastic cell growth and is required for the function of light chain amino acid transporters. It is also involved in sodium-independent transport of large neitral amino acids and in guiding and targeting LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, CD98 acts as an arginine/glutamine exchanger.
Antigen:
CD98
Concentration:
1 mg/mL
Applications:
ELISA, Immunofluorescence, Blocking Assay
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Biologically Active Proteins, Cell Biology, Cellular Markers, Immunology, Lipid and Metabolism, Plasma Membrane Markers, Signal Transduction
Formulation:
PBS
Gene ID (Entrez):
6520
Immunogen:
HBJ127 was obtained from hybridomas generated by the fusion of mouse myeloma cells and spleen cells from mice which had been immunized with T24 hyman bladder cancer cells.
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone:
HBJ127
Dilution:
ELISA, Immunocytochemistry/Immunofluorescence, Block/Neutralize
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
4F2, 4F2hc, 4T2HC, antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC, CD98, CD98 antigen, CD98 heavy chain, CD98HC, heavy chain, Lymphocyte activation antigen 4F2 large subunit, MDU1antigen defined by monoclonal 4F2, heavy chain, monoclonal 44D7, NACAE4F2 cell-surface antigen heavy chain, solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen
Gene Symbols:
SLC3A2
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
HBJ127 binds to the CD98 heavy chain at an epitope consisting of residues AFS (aa 442-444) (Itoh et al, 2007). The antibody has not been shown to cross-react with other species. CD98 is expressed at high levels on monocytes and at very low level on peripheral blood T and B lymphocytes, splenocytes, NK cells and granulocytes. CD98 is a disulfide-linked and glycosylated type II integral membrane protein. This protein has roles in normal and neoplastic cell growth and is required for the function of light chain amino acid transporters. It is also involved in sodium-independent transport of large neitral amino acids and in guiding and targeting LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, CD98 acts as an arginine/glutamine exchanger.