PKR Mouse anti-Human, Unlabeled, Clone: 13, BD

Description

• Double stranded RNA (dsRNA) generated by most viruses during the infectious cycle is a potent stimulator of interferons
• Interferons bind to cell surface receptors and stimulate the synthesis of several proteins that interfere with viral replication
• One protein, 2′5′-oligo-adenylate synthetase, indirectly activates an endoribonuclease that degrades viral RNA
• Another protein, p68 serine/threonine protein kinase (also known as PKR and TIK), is induced following interferon stimulation and activated by autophosphorylation in the presence of dsRNA
• Upon activation, p68 phosphorylates the α subunit of the eukaryotic initiation factor 2 (eIF-2) resulting in inhibition of protein synthesis and, in turn, inhibition of viral replication
• Evidence also suggests that p68 protein kinase inhibits proliferation and potentiates tumor suppressor function
• In addition, p68 has been shown to phosphorylate Iκ-B, thus activating NF-κB which induces interferon-β gene transcription
• Immunofluorescence, Immunoprecipitation, Western Blotting