p120 Catenin (pY228) Mouse, Unlabeled, Clone: 21A, BD

Description

• The membrane associated protein pp120 Src substrate (p120 Catenin, p120cas)was identified as a tyrosine kinase substrate that is phosphorylated in Src transformed cells or in response to growth factor stimulation
• It shares structural similarity with the Drosophila Armadillo protein and the vertebrate β-catenin and γ-catenin proteins
• This similarity is evidenced by its characteristic Arm domain that is composed of 42-amino acid motif repeats
• In the cell, p120 Catenin is localized to the E-Cadherin/catenins cell adhesion complex
• Like β- and γ-catenin, p120 Catenin directly associates with the cytoplasmic C-terminus of E-Cadherin via its Arm domain and may similarly interact with other Cadherins
• It exists as four isoforms that range in size from 90-115kDa
• Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered pp120 expression contributes to malignancy due to loss of functional cell adhesions
• Multiple tyrosine residues (Y96, Y112, Y228, Y280, Y257, Y291, Y296, and Y302) in p120 Catenin are phosphorylated by Src and these phosphorylations may facilitate interaction with PTP1C/SHP-1 in response to EGF stimulation
• Thus, p120 Catenin is an Arm domain protein that interacts with both cell adhesion molecules, such as cadherins and cell signaling molecules, such as PTP1C
• Flow Cytometry, Immunofluorescence, Intracellular Staining, Western Blotting