PI3-Kinase p170 Mouse, Unlabeled, Clone: 17, BD

Description

• PI3-kinase phosphorylates the D-3 position of the inositol ring of phosphatidylinositol (PtdIns), producing PtdIns(3)P, PtdIns(3,4)P2, and PtdIns(3,4,5)P3
• PI3-Kinase is a heterodimer of an 85kDa regulatory subunit (p85) and a 110kDa catalytic subunit (p110)
• However, this heterodimer is only one member of a larger family of proteins with similarity to the p110 subunit
• These different PI3-kinase isoforms have been divided into three classes
• Class I consists of p110α and p110β which bind the p85 subunit and associate with receptor tyrosine kinases
• Class II contains 68D, cpk, and p170
• These proteins all contain a C-terminal C2 domain and phosphorylate PtdIns and PtdIns(4)P, but not PtdIns(4,5)P2
• Class III members only phosphorylate PtdIns to PtdIns(3)P and include the S
• cerevisiae Vps34p and its human homologs
• The first human Class II PI3-kinase to be described, p170, contains the characteristic C-terminal phosphoinositide-binding C2 domain also found in PKC isoforms and synaptotagmins
• In addition, expression of PI3-kinase p170 is widespread and may indicate that the protein performs a housekeeping function in cell growth and differentiation
• Immunofluorescence, Western Blotting