CD158e1 (NKB1) Mouse anti-Human, BUV395, Clone: DX9, BD Optibuild™

Description

• The DX9 monoclonal antibody specifically binds to CD158e1, also known as NKB1
• CD158e1 functions as a killer cell inhibitory receptor (KIR) and is encoded by KIR3DL1 /(Killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail)
• CD158e1 is a 70 kDa glycoprotein that belongs to the Ig superfamily
• It is expressed on a subset of natural killer cells and a small subset of T cells
• Expression of CD158e1 has been observed to vary among individuals
• KIR molecules specifically recognize a certain group of HLA class I antigens
• Interaction of CD158e1 with specific HLA-B antigen on a target cell appears to inhibit cell-mediated cytotoxicity by delivering a negative signal that prevents lymphocyte activation
• It is suggested that this MHC class I-KIR interaction works as a signaling mechanism that regulates NK and T-cell responses to antigenic challenge
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.