BAH-1 Mouse anti-Human, Brilliant Violet 650, Clone: BAH-1 (also known as BAH1), BD Optibuild™

Description

• The BAH-1 monoclonal antibody was raised against human megakaryocytic cells
• It was originally proposed that clone BAH-1 was specific for human CD110, also known as c-Mpl or TPO-R
• Data has emerged to suggest that BAH-1 antibody may not recognize CD110
• However, there is strong evidence that it recognizes an antigen that is highly expressed on human Megakaryocyte-Erythroid Progenitor (MEP) cells
• In combination with CD71, BAH-1 expression has been used to distinguish subsets of Hematopoietic Stem Cells and Myeloid Progenitors in human fetal liver, cord blood, and adult bone marrow
• BAH-1 antibody can induce megakaryocytopoiesis of both mouse and human bone marrow cells in vitro
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.