CD31 Mouse anti-Human, Brilliant Violet 650, Clone: MBC 78.2 (also known as PECAM-1.2; 1.2), BD Optibuild™

Description

• The MBC 78.2 monoclonal antibody recognizes CD31 which is also known as, Platelet endothelial cell adhesion molecule (PECAM-1), platelet GPIIa, or EndoCAM
• CD31 is a ñ130 kDa type I transmembrane glycoprotein that belongs to the Ig gene superfamily
• CD31 is comprised of an extracellular region with six IgC-like domains, a transmembrane region, and a cytoplasmic domain that contains two immunoreceptor tyrosine-based inhibitory motifs (ITIMs)
• The MBC 78.2 antibody specifically binds to an epitope located on membrane-proximal, extracellular Ig-like domain 6 of CD31
• This epitope remains expressed by activated T cells after enzymatic cleavage and shedding of a soluble extracellular CD31 fragment comprised of Ig-like domains 1 to 5 from cells
• In contrast to the MBC 78.2 antibody, the WM59 monoclonal antibody reportedly binds to the extracellular Ig-like domain 2 of CD31
• WM59 can thus bind to cells that express intact CD31 but not to cells that express a truncated form CD31 that lacks at least the membrane distal Ig-like domains 1 and 2 of CD31
• CD31 has wide tissue distribution and is expressed on platelets, monocytes, granulocytes, some T cell subsets, and at high levels on endothelial cells
• This cell adhesion molecule has been implicated in a number of cellular phenomena, including vascular wound healing
• angiogenesis, transendothelial migration of leucocytes, and the regulation of T cell responses
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.