CD321 (JAM-1) Mouse anti-Human, Brilliant Violet 650, Clone: M.Ab.F11, BD Optibuild™

Description

• The M.Ab.F11 monoclonal antibody specifically binds to CD321 which is also known as JAM-1 (Junctional adhesion molecule 1), Junctional adhesion molecule A (JAM-A), and F11 Receptor (F11R)
• CD321 is a 32-35 kDa type I transmembrane glycoprotein that includes two extracellular immunoglobulin-like domains
• CD321 is expressed on platelets, leucocytes, red blood cells, endothelial cells, epithelial cells, and various cell lines
• CD321 functions as an adhesion receptor molecule on platelets
• It also supports the tight junction formation between endothelial cells, where it may regulate the transendothelial migration of leucocytes, and epithelial cells
• M.Ab.F11 is a stimulatory antibody that can induce morphological changes, granule secretion, and aggregation in human platelets
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.