CD178 Mouse anti-Human, Brilliant Violet 786, Clone: NOK-1, BD Optibuild™

Description

• The NOK-1 monoclonal antibody specifically recognizes CD178
• Fas (CD95; APO-1) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or by Fas ligand (FasL; CD178)
• Fas belongs to the TNF (Tumor Necrosis Factor)/NGF (Nerve Growth Factor) receptor family, and is expressed in various tissues and cells including the thymus, liver, ovary and lung
• CD178 (FasL), a member of the TNF cytokine family, induces apoptosis by binding to Fas, its cell-surface receptor
• FasL may exist as either membrane bound or soluble forms and is expressed by activated T and NK cells
• FasL may also be constitutively expressed in some immunologically privileged sites, e.g., eye and testis
• Fas and FasL play an important role in the induction of apoptosis, and thus regulate a variety of immunological responses
• The NOK-1 antibody clone has been reported to recognize human FasL, recognizing both the membrane bound (FasL) and soluble (sFasL) forms
• It is reported that the epitope for NOK-1 has been mapped to the COOH-terminus of FasL, at the region implicated in Fas binding
• FasL and sFasL have been reported to migrate at reduced molecular weights of 40 and 26 kDa, respectively
• However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in the literature
• The NOK-1 antibody clone is not recommended for the Western blot application
• The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm
• BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter)
• BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm
• BV786 offers a bright choice for the 6th detector off the violet laser.