CD8b Mouse, anti-Human, R718, Clone: 2ST8.5H7, BD OptiBuild™

Description

• The 2ST8.5H7 monoclonal antibody specifically recognizes an epitope formed by the combination of CD8 alpha and beta chains
• The majority of peripheral blood CD8+ T lymphocytes expresses a CD8αβ heterodimer (32, 30 kilodaltons (kDa)), while CD8+CD16+ natural killer (NK) cells and CD8+ TCR γδ+ T lymphocytes express CD8αα homodimers
• The 2ST8.5H7 antibody can therefore be used to selectively bind to CD8+ T cells while excluding CD8+ NK cells
• CD8 binds to class I major histocompatibility (MHC) molecules, resulting in increased adhesion between the CD8+ T lymphocytes and target cells
• Binding of CD8 to class I MHC molecules enhances the activation of resting T lymphocytes
• CD8 is coupled to a protein tyrosine kinase, p56lck
• The CD8:p56lck complex can play a role in T-lymphocyte activation through mediation of the interactions between CD8 and the CD3/TCR complex
• The CD8β antigen is present on the human suppressor/cytotoxic T-lymphocyte subset
• The CD8 antigen is expressed on 19% to 48% of normal peripheral blood lymphocytes and 60% to 85% of normal thymocytes
• The 2ST8.5H7 antibody crossreacts with lymphocytes of some nonhuman primate species.