Progesterone Receptor Monoclonal Antibody (PR-AT 4.14), Invitrogen™

Description

• MA1-410 detects the progesterone receptor (PR) from human, non-human primate, bovine, rat, rabbit, and mouse tissues
• This antibody does not cross-react with androgen receptor, estrogen receptor or glucocorticoid receptor
• MA1-410 has been successfully used in Western blot, immunohistochemical and immunoprecipitation procedures
• By Western blot, this antibody detects a 94 kDa and a 120 kDa protein representing PRA and PRB, respectively from T47D whole cell lysate
• MA1-410 performs better by western blot using BSA as the blocking agent and in antibody diluent
• The MA1-410 immunogen is a synthetic peptide corresponding to the binding domain residues G(533) L P Q V Y P P Y L N Y L R P(547) of human Progesterone Receptor
• MA1-410 can be used with blocking peptide PEP-016
• The progesterone receptor (PR) is a member of the steroid family of nuclear receptors
• The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy
• PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites
• PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation
• PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus
• This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity
• In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins
• Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription
• A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.