MABE106725
ESET (SETDB1), Mouse anti-Mouse, Clone: F0808, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE106725-Each-of-1 | In Stock | ₹ 16,456.61 |
MABE106725 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
ESET (SETDB1)
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.
Gene Symbols
Setdb1;Eset;Kiaa0067
Immunogen
His-tagged recombinant fragment corresponding to 213 amino acids from the internal region of murine SETDB1.
Quantity
25 μg
Research Discipline
Epigenetics & Nuclear Function
Test Specificity
Clone F0808 specifically detects Histone-lysine N-methyltransferase SETDB1. It targets an epitope with in the internal region.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Applications
ChIP Assay, Immunoprecipitation, Western Blot
Clone
F0808
Dilution
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ESET (SETDB1) in Chromatin Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).Immunoprecipitation Analysis: A representative lot immunoprecipitated ESET (SETDB1) in Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).
Gene Alias
Histone-lysine N-methyltransferase SETDB1;EC: 2.1.1.43;ERG-associated protein with SET domain;SET domain bifurcated 1
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Mouse
Form
Purified
Related Products
Description
- Anti-ESET (SETDB1), clone F0808, Cat
- No
- MABE1067, is a highly specific mouse monoclonal antibody that targets Histone-lysine N-methyltransferase SETDB1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting
- Histone-lysine N-methyltransferase SETDB1 (UniProt: O88974; also known as EC: 2.1.1.43, ERG-associated protein with SET domain, ESET, SET domain bifurcated 1, SETDB1) is encoded by the Setdb1 (also known as Eset, Kiaa0067) gene in murine species
- SETDB1 is a ubiquitously expressed histone methyltransferase with multiple zinc-binding sites that specifically trimethylates lysine 9 of histone H3 immediately downstream of transcription start sites marked with H3K4me3 to establish the bivalent domain
- It is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by Krüppel-associated box (KRAB) zinc-finger proteins
- The H3K4/H3K9me3 signature keeps RNA polymerase II (Pol II) paused at the proximal promoter and maintains gene expression at low levels, but in a poised state that is ready for activation in lineage-committed cells
- It functions mainly in euchromatin regions, thereby playing a key role in the silencing of euchromatic genes
- It probably acts by forming a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone lysine 9 trimethylation
- SETDB1 is reported to inhibit adipogenesis and its depletion induces preadipocyte differentiation and adipogenesis in mesenchymal stem cells and preadipocytes
- Expression of Cebpa and Pparg genes that are its key targets of SETDB1 is enhanced when SETDB1 is knocked down in undifferentiated cells
- Murine SETDB1 contains two Tudor domains (aa 257-320 and 347-403) and pre-SET (aa 744-817), SET (aa 820-1282), and post-SET (aa 1291-1307) domains that are required for its methyltransferase activity
- (Ref.: Matsumura, Y., et al
- (2015)
- Mol
- Cell 60(4); 584-596).
Compare Similar Items
Show Difference
Antigen: ESET (SETDB1)
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.
Gene Symbols: Setdb1;Eset;Kiaa0067
Immunogen: His-tagged recombinant fragment corresponding to 213 amino acids from the internal region of murine SETDB1.
Quantity: 25 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: Clone F0808 specifically detects Histone-lysine N-methyltransferase SETDB1. It targets an epitope with in the internal region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Applications: ChIP Assay, Immunoprecipitation, Western Blot
Clone: F0808
Dilution: Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ESET (SETDB1) in Chromatin Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).Immunoprecipitation Analysis: A representative lot immunoprecipitated ESET (SETDB1) in Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).
Gene Alias: Histone-lysine N-methyltransferase SETDB1;EC: 2.1.1.43;ERG-associated protein with SET domain;SET domain bifurcated 1
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Mouse
Form: Purified
Antigen:
ESET (SETDB1)
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.
Gene Symbols:
Setdb1;Eset;Kiaa0067
Immunogen:
His-tagged recombinant fragment corresponding to 213 amino acids from the internal region of murine SETDB1.
Quantity:
25 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
Clone F0808 specifically detects Histone-lysine N-methyltransferase SETDB1. It targets an epitope with in the internal region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
ChIP Assay, Immunoprecipitation, Western Blot
Clone:
F0808
Dilution:
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ESET (SETDB1) in Chromatin Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).Immunoprecipitation Analysis: A representative lot immunoprecipitated ESET (SETDB1) in Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).
Gene Alias:
Histone-lysine N-methyltransferase SETDB1;EC: 2.1.1.43;ERG-associated protein with SET domain;SET domain bifurcated 1
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Mouse
Form:
Purified
Antigen: APE/Ref-1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse IgG2a in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols: __
Immunogen: Recombinant full-length human APE/Ref-1.
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: Clone 13B8E5C2 detected both cytosolic and nuclear APE/Ref-1 by Immunocytochemistry, Immunohistochemistry, and Western blotting (Moore, D.H., et al. (2000). Clin. Cancer Res. 6(2):602-609).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG2a κ
Applications: Immunohistochemistry (Paraffin), Western Blot
Clone: 13B8E5C2
Dilution: __
Gene Alias: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
APE/Ref-1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse IgG2a in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols:
__
Immunogen:
Recombinant full-length human APE/Ref-1.
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
Clone 13B8E5C2 detected both cytosolic and nuclear APE/Ref-1 by Immunocytochemistry, Immunohistochemistry, and Western blotting (Moore, D.H., et al. (2000). Clin. Cancer Res. 6(2):602-609).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG2a κ
Applications:
Immunohistochemistry (Paraffin), Western Blot
Clone:
13B8E5C2
Dilution:
__
Gene Alias:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Antigen: BAP1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: __
Gene Symbols: BAP1,KIAA0272,hucep-6
Immunogen: GST-tagged full length recombinant human ubiquitin carboxyl-terminal hydrolase BAP1.
Quantity: 200 μL
Research Discipline: Epigenetics and Nuclear Function
Test Specificity: Clone 3C11 specifically detects BAP1 in human. It targets an epitope within amino acids 337-440 in human BAP1.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Applications: Immunoprecipitation, Western Blot
Clone: 3C11
Dilution: __
Gene Alias: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
BAP1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
__
Gene Symbols:
BAP1,KIAA0272,hucep-6
Immunogen:
GST-tagged full length recombinant human ubiquitin carboxyl-terminal hydrolase BAP1.
Quantity:
200 μL
Research Discipline:
Epigenetics and Nuclear Function
Test Specificity:
Clone 3C11 specifically detects BAP1 in human. It targets an epitope within amino acids 337-440 in human BAP1.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
Immunoprecipitation, Western Blot
Clone:
3C11
Dilution:
__
Gene Alias:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Antigen: BAP1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols: __
Immunogen: Recombinant full-length human BAP1.
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: Clone 1G8 targets human BAP1 UCH domain.
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG1 κ
Applications: Western Blot
Clone: 1G8
Dilution: __
Gene Alias: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
BAP1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols:
__
Immunogen:
Recombinant full-length human BAP1.
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
Clone 1G8 targets human BAP1 UCH domain.
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
Western Blot
Clone:
1G8
Dilution:
__
Gene Alias:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified