TLR4 Monoclonal Antibody (HTA125), PE, Invitrogen™

Description

• This antibody has been demonstrated to block activation of monocytes with LPS
• TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity
• TLRs are highly conserved from Drosophila to humans and share structural and functional similarities
• This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes
• Mammalian cells respond to LPS by activating TLR4
• TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria
• TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity
• The various TLRs exhibit different patterns of expression
• Mutations in the TLR4 gene have been associated with differences in LPS responsiveness
• Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain
• TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta
• Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide
• Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.