HIF-1 beta Recombinant Rabbit Monoclonal Antibody (ST05-25), Invitrogen™

Description

• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis
• This occurs as a posttranslational modification by prolyl hydroxylation
• HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits
• Both subunits are constantly translated
• However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes
• Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity
• Therefore, the concentration of HIF-1 alpha increases in the cell
• In contrast, HIF-1 beta remains stable under either condition
• HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis
• This occurs as a posttranslational modification by prolyl hydroxylation
• HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits
• Both subunits are constantly translated
• However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes
• Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity
• Therefore, the concentration of HIF-1 alpha increases in the cell
• In contrast, HIF-1 beta remains stable under either condition
• HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.