F2518
Human Fibronectin
from human foreskin fibroblasts, powder, suitable for cell culture
Manufacturer: Sigma Aldrich
Synonym(S): Fibronectin
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.5 MG | F2518-0.5-MG | In Stock | ₹ 1,00,625.60 |
F2518 - 0.5 MG
In Stock
Quantity
1
Base Price: ₹ 1,00,625.60
GST (18%): ₹ 18,112.608
Total Price: ₹ 1,18,738.208
product name
Fibronectin human foreskin fibroblasts, lyophilized powder, suitable for cell culture
biological source
human foreskin fibroblasts
Quality Level
200
product line
BioReagent
form
lyophilized powder
mol wt
~550 kDa
packaging
pkg of 0.5 mg
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑5 μg/cm2
impurities
Human Source, none detected (HCV) Human Source, none detected (HIV) Human Source, none detected (HTLV) Human Source, none detected (HbSAg) Microbial Contamination, passes test
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Description
- General description: Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35.[1] Fibronectin is mostly synthesized by fibroblasts and endothelial cells.[2]It is an adhesive glycoprotein,[3] predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.[4]
- Application: Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells
- Biochem/physiol Actions: Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis.[1] Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis.[10] Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.[2]
- Features and Benefits: Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.The starting material is tested and found negative for HIV and HBsAg.The product is soluble in sterile water, yielding a clear, colorless solution.It can be stored at -20°C for at least three years.
- Caution: Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.
- Physical form: Lyophilized in CAPS buffered saline
- Preparation Note: dialyzed against chloroform
- Reconstitution: Store reconstituted solution in working aliquots at -20°C or lower.
- Analysis Note: Protein homogeneity is evaluated by immunoelectrophoresis.
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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product name: Fibronectin human foreskin fibroblasts, lyophilized powder, suitable for cell culture
biological source: human foreskin fibroblasts
Quality Level: 200
product line: BioReagent
form: lyophilized powder
mol wt: ~550 kDa
packaging: pkg of 0.5 mg
technique(s): cell culture | mammalian: suitable
surface coverage: 1‑5 μg/cm2
impurities: Human Source, none detected (HCV) Human Source, none detected (HIV) Human Source, none detected (HTLV) Human Source, none detected (HbSAg) Microbial Contamination, passes test
product name:
Fibronectin human foreskin fibroblasts, lyophilized powder, suitable for cell culture
biological source:
human foreskin fibroblasts
Quality Level:
200
product line:
BioReagent
form:
lyophilized powder
mol wt:
~550 kDa
packaging:
pkg of 0.5 mg
technique(s):
cell culture | mammalian: suitable
surface coverage:
1‑5 μg/cm2
impurities:
Human Source, none detected (HCV) Human Source, none detected (HIV) Human Source, none detected (HTLV) Human Source, none detected (HbSAg) Microbial Contamination, passes test
product name: __
biological source: corn
Quality Level: 200
product line: BioXtra
form: powder
mol wt: __
packaging: __
technique(s): HPLC: suitable
surface coverage: __
impurities: <0.0005% Phosphorus (P)<0.05% glucose (enzymatic)<0.1% Insoluble matter
product name:
__
biological source:
corn
Quality Level:
200
product line:
BioXtra
form:
powder
mol wt:
__
packaging:
__
technique(s):
HPLC: suitable
surface coverage:
__
impurities:
<0.0005% Phosphorus (P)<0.05% glucose (enzymatic)<0.1% Insoluble matter
product name: __
biological source: corn
Quality Level: 200
product line: BioXtra
form: powder
mol wt: __
packaging: __
technique(s): HPLC: suitable
surface coverage: __
impurities: <0.0005% Phosphorus (P)<0.05% glucose (enzymatic)<0.1% Insoluble matter
product name:
__
biological source:
corn
Quality Level:
200
product line:
BioXtra
form:
powder
mol wt:
__
packaging:
__
technique(s):
HPLC: suitable
surface coverage:
__
impurities:
<0.0005% Phosphorus (P)<0.05% glucose (enzymatic)<0.1% Insoluble matter
product name: __
biological source: rabbit
Quality Level: 200
product line: __
form: __
mol wt: __
packaging: __
technique(s): immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100indirect ELISA: suitablewestern blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein
surface coverage: __
impurities: __
product name:
__
biological source:
rabbit
Quality Level:
200
product line:
__
form:
__
mol wt:
__
packaging:
__
technique(s):
immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100indirect ELISA: suitablewestern blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein
surface coverage:
__
impurities:
__