Insulin-like Growth Factor-I human

Description

• General description: Insulin-like growth factor I (IGF-I) is localized on human chromosome 12q23.2.[1]
• Application: Insulin-like Growth Factor-I human has been used:in modified Dulbecco minimal essential medium for in vitro culture of testicular cells[2][3]in culture media, to study its effect on steroidogenesis during testicular development[4]in culture media for in vitro assay of splenic pro-erythroid cell growth and differentiation[5]
• Biochem/physiol Actions: IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.
• Physical form: Lyophilized from 0.2 μm filtered solution in 50 mM acetic acid.
• Reconstitution: Reconstitute in water
• Analysis Note: The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED50 corresponds to a specific activity of at least 1 x 105 units/mg.