SSPE Buffer

Description

• Application: Saline-Sodium Phosphate-EDTA is used as a hybridization buffer in Northern and Southern blotting. It is also used as a wash buffer in DNA-microarray technique.
• Features and Benefits: Ready-to-use bufferNo measuring and pH adjusting neededSave time and minimize effortBiological tests: free of DNase, RNase, protease, and nickaseChemical tests: Iron ≤10 ppm, lead ≤5 ppm
• Packaging: Foil pouches
• Reconstitution: Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl, 10 mM NaH2PO4, 1 mM EDTA, pH 7.4 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
• Storage and Stability: Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
• Other Notes: For additional information on our range of Biochemicals, please complete this form.
• Legal Information: pHast Pack is a trademark of Sigma-Aldrich Co. LLC