Bis-Tris Bicine EDTA buffer

Description

• General description: Bis-Tris Bicine EDTA buffer or Bis-Tris buffer (pH 7.2) contains Bis-Tris and Bicine, zwitterionic buffer components that form strong bonds with common metals. In combination with ethylenediaminetetraacetic acid (EDTA), a metal chelator, that binds divalent cations. This buffer is commonly used as a transfer buffer in western blot to transfer proteins from the gel to the membrane.
• Application: Bis-Tris buffer may be used in western blot as a transfer buffer.
• Features and Benefits: Ready-to-use buffer No measuring and pH adjustment needed Certified quality for reproducibility Minimizes exposure to toxic chemicals Biological tests: free of DNase, RNase, Protease, and Nickase Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm
• Packaging: Foil pouches
• Reconstitution: Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1X solution containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, pH 7.2 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
• Storage and Stability: Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
• Other Notes: For additional information on our range of Biochemicals, please complete this form.
• Legal Information: pHast Pack is a trademark of Sigma-Aldrich Co. LLC