11-750-350
Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-750-350-Each-of-1 | In Stock | ₹ 5,32,183.20 |
11-750-350 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 5,32,183.20
GST (18%): ₹ 95,792.976
Total Price: ₹ 6,27,976.176
Content And Storage
• SuperScript IV CellsDirect Lysis Solution (3 x 9 mL)• SuperScript IV CellsDirect Stop Solution (2 x 1.25 mL)• Lysis Enhancer (1 x 250 μL)• DNase I (1 x 500 μL)• SuperScript IV RT Master Mix (4 x 1 mL)• SuperScript IV No RT Control (4 x 1 mL)Store at –20°C.
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Optimal Reaction Temperature
50°C
Ribonuclease H Activity
Reduced
For Use With (Application)
RT-PCR, Real Time PCR (qPCR)
No. of Reactions
500 Reactions
Reagent Type
Reverse Transcription
Format
Kit
Reaction Speed
10 min.
Green Features
Less Hazardous, Less Waste
Reverse Transcriptase
SuperScript IV
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reaction Format
Premixed Components
Starting Material
Cells
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Description
- The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA
- Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR
- Features of the SuperScript IV CellsDirect Synthesis Kit • Compatible with a wide range of mammalian cell types grown under different treatment conditions • Single-tube format minimizes reagent loss, sample loss, and handling time • Total lysate volume can be used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts • High-quality cDNA generated for use in a variety of applications, including PCR and qPCR • Simple protocol takes approximately 37 minutes • Compatible with TaqMan and SYBR Green qPCR master mixes from various manufacturers • Optimized performance for 1–10,000 cells per sample, results equivalent to those from purified RNA Features of the SuperScript IV Reverse Transcriptase included in this kit • Reduced RNase H activity • High thermal stability • High yields of cDNA in the first-strand synthesis reaction for greater sensitivity and enhanced detection of rare transcripts • Superior tolerance to various RT inhibitors SuperScript IV reverse transcriptase is a top choice for all RT-PCR and qRT-PCR applications
- It is a proprietary MMLV mutant with superior robustness and reliability in RT reactions
- It is significantly improved over SuperScript III reverse transcriptase in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of the previous enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity
- How it works In traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material
- Using the SuperScript IV CellsDirect cDNA Synthesis Kit, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss
- DNase I treatment is an optional step and can be used to eliminate genomic DNA prior to first-strand synthesis
- This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell.
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Content And Storage: • SuperScript IV CellsDirect Lysis Solution (3 x 9 mL)• SuperScript IV CellsDirect Stop Solution (2 x 1.25 mL)• Lysis Enhancer (1 x 250 μL)• DNase I (1 x 500 μL)• SuperScript IV RT Master Mix (4 x 1 mL)• SuperScript IV No RT Control (4 x 1 mL)Store at –20°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
For Use With (Application): RT-PCR, Real Time PCR (qPCR)
No. of Reactions: 500 Reactions
Reagent Type: Reverse Transcription
Format: Kit
Reaction Speed: 10 min.
Green Features: Less Hazardous, Less Waste
Reverse Transcriptase: SuperScript IV
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Premixed Components
Starting Material: Cells
Content And Storage:
• SuperScript IV CellsDirect Lysis Solution (3 x 9 mL)• SuperScript IV CellsDirect Stop Solution (2 x 1.25 mL)• Lysis Enhancer (1 x 250 μL)• DNase I (1 x 500 μL)• SuperScript IV RT Master Mix (4 x 1 mL)• SuperScript IV No RT Control (4 x 1 mL)Store at –20°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
RT-PCR, Real Time PCR (qPCR)
No. of Reactions:
500 Reactions
Reagent Type:
Reverse Transcription
Format:
Kit
Reaction Speed:
10 min.
Green Features:
Less Hazardous, Less Waste
Reverse Transcriptase:
SuperScript IV
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Premixed Components
Starting Material:
Cells
Content And Storage: • SuperScript IV CellsDirect Lysis Solution (9 mL)• SuperScript IV CellsDirect Stop Solution (1.25 mL)• Lysis Enhancer (250 μL)Store at –20°C.
GC-Rich PCR Performance: __
Technique: Reverse Transcription
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): __
No. of Reactions: 500 reactions
Reagent Type: Reverse Transcription
Format: Kit
Reaction Speed: __
Green Features: __
Reverse Transcriptase: SuperScript IV
Shipping Condition: Dry Ice
Final Product Type: __
Reaction Format: Master Mix
Starting Material: Cells
Content And Storage:
• SuperScript IV CellsDirect Lysis Solution (9 mL)• SuperScript IV CellsDirect Stop Solution (1.25 mL)• Lysis Enhancer (250 μL)Store at –20°C.
GC-Rich PCR Performance:
__
Technique:
Reverse Transcription
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
__
No. of Reactions:
500 reactions
Reagent Type:
Reverse Transcription
Format:
Kit
Reaction Speed:
__
Green Features:
__
Reverse Transcriptase:
SuperScript IV
Shipping Condition:
Dry Ice
Final Product Type:
__
Reaction Format:
Master Mix
Starting Material:
Cells
Content And Storage: • 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription, Preamplification
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: __
For Use With (Application): Next-Generation Sequencing (NGS), Real Time PCR (qPCR)
No. of Reactions: 48 Reactions
Reagent Type: Reverse Transcription
Format: Kit
Reaction Speed: 30 min.
Green Features: __
Reverse Transcriptase: SuperScript IV
Shipping Condition: Dry Ice
Final Product Type: PCR Amplified cDNA
Reaction Format: __
Starting Material: Cells or RNA
Content And Storage:
• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription, Preamplification
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
__
For Use With (Application):
Next-Generation Sequencing (NGS), Real Time PCR (qPCR)
No. of Reactions:
48 Reactions
Reagent Type:
Reverse Transcription
Format:
Kit
Reaction Speed:
30 min.
Green Features:
__
Reverse Transcriptase:
SuperScript IV
Shipping Condition:
Dry Ice
Final Product Type:
PCR Amplified cDNA
Reaction Format:
__
Starting Material:
Cells or RNA
Content And Storage: • 2X RT Reaction Mix (500 μL)• RT Enzyme Mix (100 μL)•E. coliRNase (50 μL)Store at –20°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: __
For Use With (Application): Real Time PCR (qPCR)
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Format: Super Mix
Reaction Speed: 30 min.
Green Features: __
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Starting Material: RNA
Content And Storage:
• 2X RT Reaction Mix (500 μL)• RT Enzyme Mix (100 μL)•E. coliRNase (50 μL)Store at –20°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
__
For Use With (Application):
Real Time PCR (qPCR)
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Format:
Super Mix
Reaction Speed:
30 min.
Green Features:
__
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Starting Material:
RNA