PI16197
Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI16197-Each-of-1 | In Stock | ₹ 88,126.80 |
PI16197 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 88,126.80
GST (18%): ₹ 15,862.824
Total Price: ₹ 1,03,989.624
Type
Assay Kit
Sufficient For
1000 Luciferase Assays in Microplate Wells
Content And Storage
• Firefly Luc One-Step Substrate, Lyophilized (makes 1 mL of 1X), store at -20°C• Firefly Luc One-Step Assay Buffer, 115 mL, store at -20°CUpon receipt store kit at -20°C.
Compatible Cells
Mammalian Cells
For Use With (Equipment)
Luminometer (Microplate)
Label Type
Enzyme Labeled
Technique
Enhanced Chemiluminescence
Substrate
D-Luciferin
Target
Luciferase, Firefly Luciferase, Cypridina Luciferase
Quantity
1000 Reaction Kit
Assay
Reporter Enzyme, Luciferase Reporter Assay
Detection Method
Bioluminescence
Format
384-well plate, 96-well plate
Substrate Properties
Chemical Substrate
Substrate Type
Luciferase Substrate
Related Products
Description
- The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications
- This glow-type luciferase assay kit is designed for use with cultured mammalian cells that are transfected with vectors expressing the appropriate firefly luciferase reporter enzyme
- Several features make the kit especially suitable for luminometers without injectors and for high-throughput screening (HTS) applications
- The convenient, one-step, homogenous protocol minimizes handling steps to support the use of automation
- Simply combine the supplied substrate solution and assay buffer to make a single working solution, and then add it to microplate wells containing the transfected, treated cells
- The stable signal output (glow) of this system provides flexibility with regard to incubation time
- When used with Thermo Scientific Firefly Luc Plasmids, the kit provides a highly sensitive bioluminescent reporter assay system for the detection of promoter or pathway activity
- Highlights: Sensitive - highly sensitive detection of firefly luciferase activity Convenient - one-step, mix-and-read protocol; no additional reagents required Stable - increased signal half-life and stability compared to flash assays Simple - does not require a luminometer with injectors Automation-friendly - amenable to high throughput assays Includes: Assay buffer and substrate solution (100X) Requires: Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers
- Applications: Promoter studies for analyzing cis- and trans-acting regulatory elements Drug screening siRNA and miRNA screening Multiplexed assays to study off-target effects Protein localization reporter assays Signal transduction pathway analysis Product Details: Firefly luciferase is a 62kDa protein produced in nature by several species of the Lampyridae family of beetles, which includes the genera Photinus and Luciola
- Thermo Scientific Red Firefly Luciferase has an emission range of 560-700nm (λmax = 613nm)
- Bioluminescent signal from firefly luciferase originates from the oxidation of D-Luciferin
- Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly luciferase expression
- Firefly luciferase bioluminescence provides researchers with a non-radioactive assay for measuring transcriptional activity of regulatory elements
- Firefly luciferase reporter activity can be used to determine promoter activity, to study upstream pathway activity in the cell, or to determine effect of activators or inhibitors.
Compare Similar Items
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Type: Assay Kit
Sufficient For: 1000 Luciferase Assays in Microplate Wells
Content And Storage: • Firefly Luc One-Step Substrate, Lyophilized (makes 1 mL of 1X), store at -20°C• Firefly Luc One-Step Assay Buffer, 115 mL, store at -20°CUpon receipt store kit at -20°C.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Substrate: D-Luciferin
Target: Luciferase, Firefly Luciferase, Cypridina Luciferase
Quantity: 1000 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Type:
Assay Kit
Sufficient For:
1000 Luciferase Assays in Microplate Wells
Content And Storage:
• Firefly Luc One-Step Substrate, Lyophilized (makes 1 mL of 1X), store at -20°C• Firefly Luc One-Step Assay Buffer, 115 mL, store at -20°CUpon receipt store kit at -20°C.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Substrate:
D-Luciferin
Target:
Luciferase, Firefly Luciferase, Cypridina Luciferase
Quantity:
1000 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Type: __
Sufficient For: __
Content And Storage: Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA)• Heptanucleotide Mix (5X), 100 μL• Deoxynucleotide Mix (5X), 80 μL• Reaction Buffer (10X), 50 μL• DNA Polymerase Klenow Fragment (5U/μL), 10 μL• Control DNA (250 ng/μL), 5 μL• Ammonium Acetate (5M), 1 mL• EDTA (0.5M, pH 8.0), 1 mL• Nuclease-free Water, 1 mL• Biotin-11-dUTP, 20 μLStore all components at -20°C.
Compatible Cells: __
For Use With (Equipment): __
Label Type: __
Technique: __
Substrate: __
Target: __
Quantity: 10 Reactions
Assay: __
Detection Method: __
Format: Kit
Substrate Properties: __
Substrate Type: __
Type:
__
Sufficient For:
__
Content And Storage:
Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA)• Heptanucleotide Mix (5X), 100 μL• Deoxynucleotide Mix (5X), 80 μL• Reaction Buffer (10X), 50 μL• DNA Polymerase Klenow Fragment (5U/μL), 10 μL• Control DNA (250 ng/μL), 5 μL• Ammonium Acetate (5M), 1 mL• EDTA (0.5M, pH 8.0), 1 mL• Nuclease-free Water, 1 mL• Biotin-11-dUTP, 20 μLStore all components at -20°C.
Compatible Cells:
__
For Use With (Equipment):
__
Label Type:
__
Technique:
__
Substrate:
__
Target:
__
Quantity:
10 Reactions
Assay:
__
Detection Method:
__
Format:
Kit
Substrate Properties:
__
Substrate Type:
__
Type: __
Sufficient For: __
Content And Storage: Sufficient For: 1000 cm2of membrane (10 mini-gel blots)• Stabilized Streptavidin-HRP, 1.5 mL• Luminol/Enhancer Solution, 80 mL• Stable Peroxide Solution, 80 mL• Hybridization Buffer, 125 mL• Hybridization Stringency Wash Buffer (2X), 375 mL• Blocking Buffer, 500 mL• Wash Buffer (4X), 500 mL• Substrate Equilibration Buffer, 500 mLUpon receipt, store kit at 4°C.
Compatible Cells: __
For Use With (Equipment): __
Label Type: __
Technique: __
Substrate: __
Target: __
Quantity: 2160 mL Kit
Assay: __
Detection Method: Chromogenic
Format: Kit
Substrate Properties: __
Substrate Type: __
Type:
__
Sufficient For:
__
Content And Storage:
Sufficient For: 1000 cm2of membrane (10 mini-gel blots)• Stabilized Streptavidin-HRP, 1.5 mL• Luminol/Enhancer Solution, 80 mL• Stable Peroxide Solution, 80 mL• Hybridization Buffer, 125 mL• Hybridization Stringency Wash Buffer (2X), 375 mL• Blocking Buffer, 500 mL• Wash Buffer (4X), 500 mL• Substrate Equilibration Buffer, 500 mLUpon receipt, store kit at 4°C.
Compatible Cells:
__
For Use With (Equipment):
__
Label Type:
__
Technique:
__
Substrate:
__
Target:
__
Quantity:
2160 mL Kit
Assay:
__
Detection Method:
Chromogenic
Format:
Kit
Substrate Properties:
__
Substrate Type:
__
Type: __
Sufficient For: __
Content And Storage: Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA) and 10 mini-gel blots• North2South Chemiluminescent Hybridization and Detection Kit (Part No. 17097), 1 kit• North2South Biotin Random Prime Labeling Kit (Part No. 17075), 1 kit
Compatible Cells: __
For Use With (Equipment): __
Label Type: __
Technique: __
Substrate: __
Target: __
Quantity: 2164 mL Kit
Assay: __
Detection Method: Chromogenic
Format: Kit
Substrate Properties: __
Substrate Type: __
Type:
__
Sufficient For:
__
Content And Storage:
Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA) and 10 mini-gel blots• North2South Chemiluminescent Hybridization and Detection Kit (Part No. 17097), 1 kit• North2South Biotin Random Prime Labeling Kit (Part No. 17075), 1 kit
Compatible Cells:
__
For Use With (Equipment):
__
Label Type:
__
Technique:
__
Substrate:
__
Target:
__
Quantity:
2164 mL Kit
Assay:
__
Detection Method:
Chromogenic
Format:
Kit
Substrate Properties:
__
Substrate Type:
__