Thermo Scientific™ TurboLuc™ Luciferase One-Step Glow Assay Kit

Description

• The Thermo Scientific TurboLuc Luciferase One-Step Glow Assay Kit measures TurboLuc16 (Tluc16) luciferase activity in mammalian cells with a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications
• Sensitive - extremely bright, Tluc16 luciferase allows detection at a single copy per cell Increased dynamic range - optimized reagents result in higher signal-to-noise ratio Stable signal - less than 40% signal loss over 60 min duration Convenient - one-step, mix-and-read, scalable protocol Automation-friendly - amenable to HTS protocols Simple - does not require a luminometer with injectors This glow-type luciferase assay kit is designed for use with cultured mammalian cells expressing the Tluc16 luciferase reporter enzyme
• Tluc16 is a 16 kDa, novel intracellular luciferase derived from the marine copedod Metridia luciferase family
• The wild type luciferase has been modified to reduce its size, increase its brightness, enable its intracellular expression, and optimize it for HTS
• A series of vectors with and without promoters are available for expression of Tluc16 luciferase reporter in mammalian cells
• Several features of the TurboLuc Luciferase One-Step Glow Assay Kit make it suitable for luminometers without injectors and for HTS applications
• The convenient, one-step, homogenous protocol minimizes handling steps to support the use of automation
• Simply combine the supplied substrate solution and assay buffer to make a single working solution, and then add it to microplate containing the transfected, treated cells
• The stable signal output (glow enzyme kinetics) of this system provides flexibility with regard to lead time before read
• When used with Thermo Scientific TurboLuc 16 luciferase vectors, the kit provides a highly sensitive bioluminescent reporter assay system for the detection of promoter or pathway activity
• Requires: Tluc16 luciferase assay system and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan™ Ascent and Varioskan™ Flash microplate readers
• Applications: Gene reporter assays Promoter studies for analyzing cis- and trans-acting regulatory elements Drug screening Multiplexed assays to study off-target effects Cell signaling or signal transduction pathway analysis