ABE1462MI
MilliporeSigma™ phospho LSD1 (Ser112), Rabbit, Unlabeled, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABE1462MI-Each-of-1 | In Stock | ₹ 38,734.72 |
ABE1462MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,734.72
GST (18%): ₹ 6,972.25
Total Price: ₹ 45,706.97
Antigen
phospho LSD1 (Ser112)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
Kdm1a, Aof2, Kiaa0601, Lsd1
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
The Ser112 designation is based on murine KDM1A/LSD1 sequence (UniProt Q6ZQ88). Murine Ser112 is equivalent to human Ser111 and rat Ser106 (UniProt O60341 & F1MA31). Specificity of this polyclonal antibody was confirmed by dot blot analysis using the immunogen peptide and the corresponding non-phosphorylated peptide, as well as by Western blotting analysis of PMA-induced target phosphorylation, lambda phosphatase treatment of the cell lysate prior to Western blotting completely abolished the target band detection (Nam, H.J., et al. (2014). Mol. Cell 53:791-805).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Applications
Western Blot
Concentration
Please refer to lot specific datasheet.
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
Q6ZQ88
Immunogen
Linear peptide corresponding to a mouse LSD1 sequence containing the phosphorylated Ser112.
Quantity
100 μL
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_598633
Target Species
Human
Related Products
Description
- Lysine-specific histone demethylase 1A (UniProt Q6ZQ88; also known as BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2) is encoded by the Kdm1a (also known as Aof2, Kiaa0601, Lsd1) gene (Gene ID 99982) in murine species
- The FAD+-dependent, lysine-specific demethylase (LSD) LSD1 is a component of the REST corepressor (CoREST) complexes that mediate gene repression by catalyzing the demethylation of mono- and di-methylated histone H3 Lys4 (H3K4me1/2)
- LSD1 is also involved in gene activation process associated with nuclear receptors through demethylation of H3K9me1/2
- In addition, LSD1 is known to demethylate non-histone substrates, such as p53 and Dnmt1
- LSD1 is reported to be phosphorylated on Ser112 by PKCalpha and thereby mediate PKCalpha signaling-dependent circadian clock regulation
- Ser112 phosphorylation induces LSD1 interaction with CLOCK:BMAL1 to facilitate E-box-dependent transcriptional activation
- Knockin mice bearing Lsd1(SA/SA) alleles coding for phosphorylation-defective S112A LSD1 mutant are reported to exhibit altered circadian rhythms in locomotor behavior with attenuated rhythmic expression of core clock genes and impaired circadian clock phase resetting.
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Antigen: phospho LSD1 (Ser112)
Classification: Polyclonal
Conjugate: Unconjugated
Gene: Kdm1a, Aof2, Kiaa0601, Lsd1
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: The Ser112 designation is based on murine KDM1A/LSD1 sequence (UniProt Q6ZQ88). Murine Ser112 is equivalent to human Ser111 and rat Ser106 (UniProt O60341 & F1MA31). Specificity of this polyclonal antibody was confirmed by dot blot analysis using the immunogen peptide and the corresponding non-phosphorylated peptide, as well as by Western blotting analysis of PMA-induced target phosphorylation, lambda phosphatase treatment of the cell lysate prior to Western blotting completely abolished the target band detection (Nam, H.J., et al. (2014). Mol. Cell 53:791-805).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Applications: Western Blot
Concentration: Please refer to lot specific datasheet.
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.: Q6ZQ88
Immunogen: Linear peptide corresponding to a mouse LSD1 sequence containing the phosphorylated Ser112.
Quantity: 100 μL
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_598633
Target Species: Human
Antigen:
phospho LSD1 (Ser112)
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
Kdm1a, Aof2, Kiaa0601, Lsd1
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
The Ser112 designation is based on murine KDM1A/LSD1 sequence (UniProt Q6ZQ88). Murine Ser112 is equivalent to human Ser111 and rat Ser106 (UniProt O60341 & F1MA31). Specificity of this polyclonal antibody was confirmed by dot blot analysis using the immunogen peptide and the corresponding non-phosphorylated peptide, as well as by Western blotting analysis of PMA-induced target phosphorylation, lambda phosphatase treatment of the cell lysate prior to Western blotting completely abolished the target band detection (Nam, H.J., et al. (2014). Mol. Cell 53:791-805).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Applications:
Western Blot
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.:
Q6ZQ88
Immunogen:
Linear peptide corresponding to a mouse LSD1 sequence containing the phosphorylated Ser112.
Quantity:
100 μL
Research Discipline:
Epigenetics & Nuclear Function
Gene ID (Entrez):
NP_598633
Target Species:
Human
Antigen: monomethyl Histone H2B (Pro1)
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This rabbit polyclonal antibody specifically detect Histone H2B monomethylated on proline 1.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Applications: Western Blot, Inhibition Assays
Concentration: Please refer to lot specific datasheet.
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Accession No.: __
Immunogen: KLH-conjugated linear peptide corresponding to the first 9 amino acids (methiionine removed) from the N-terminus region of human Histone H2B1C with methylated proline.
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_003509
Target Species: Human
Antigen:
monomethyl Histone H2B (Pro1)
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This rabbit polyclonal antibody specifically detect Histone H2B monomethylated on proline 1.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Applications:
Western Blot, Inhibition Assays
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Accession No.:
__
Immunogen:
KLH-conjugated linear peptide corresponding to the first 9 amino acids (methiionine removed) from the N-terminus region of human Histone H2B1C with methylated proline.
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Gene ID (Entrez):
NP_003509
Target Species:
Human
Antigen: monomethyl Histone H2B (Pro1)
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This rabbit polyclonal antibody specifically detect Histone H2B monomethylated on proline 1.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Applications: Western Blot, Inhibition Assays
Concentration: Please refer to lot specific datasheet.
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Accession No.: __
Immunogen: KLH-conjugated linear peptide corresponding to the first 9 amino acids (methiionine removed) from the N-terminus region of human Histone H2B1C with methylated proline.
Quantity: 25 μg
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_003509
Target Species: Human
Antigen:
monomethyl Histone H2B (Pro1)
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This rabbit polyclonal antibody specifically detect Histone H2B monomethylated on proline 1.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Applications:
Western Blot, Inhibition Assays
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Accession No.:
__
Immunogen:
KLH-conjugated linear peptide corresponding to the first 9 amino acids (methiionine removed) from the N-terminus region of human Histone H2B1C with methylated proline.
Quantity:
25 μg
Research Discipline:
Epigenetics & Nuclear Function
Gene ID (Entrez):
NP_003509
Target Species:
Human
Antigen: phospho Histone H3.3 (Thr32)
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: __
Primary or Secondary: Primary
Test Specificity: __
Content And Storage: 2-8°C for 12 months
Applications: Dot Blot, Immunoprecipitation, Western Blot
Concentration: __
Formulation: Purified rabbit polyclonal serum in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.: __
Immunogen: KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3.3 phosphorylated at Thr32.
Quantity: 100 μg
Research Discipline: __
Gene ID (Entrez): NP_002098
Target Species: Human
Antigen:
phospho Histone H3.3 (Thr32)
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
__
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
2-8°C for 12 months
Applications:
Dot Blot, Immunoprecipitation, Western Blot
Concentration:
__
Formulation:
Purified rabbit polyclonal serum in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.:
__
Immunogen:
KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3.3 phosphorylated at Thr32.
Quantity:
100 μg
Research Discipline:
__
Gene ID (Entrez):
NP_002098
Target Species:
Human