ABE290
MilliporeSigma™ TET3, Rabbit, Unlabeled, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABE290-Each-of-1 | In Stock | ₹ 37,728.54 |
ABE290 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 37,728.54
GST (18%): ₹ 6,791.137
Total Price: ₹ 44,519.677
Antigen
TET3
Classification
Polyclonal
Conjugate
Unconjugated
Gene
TET3, KIAA0401
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Immunogen sequence is present in TET3 isoforms Tet3FL, Tet3s, and Tet3o spliced isoforms reported by Jin, S.G., et al. (2016). Cell Rep. 14(3):493-505), as well as all additional human and mouse TET3 spliced isoforms reported by UniProt (O43151, Q8BG87). Note that UniProt isoform 1 (O43151-1/human and Q8BG87-1/mouse) corresponds to isoform Tet3s, not Tet3FL (NM_001287491.1/human and JX946278.1/mouse).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt. Note: It is recommended to store the product undiluted at 2°-8°C and protected from prolonged exposure to light. Do not freeze.
Applications
ChIP Assay, Immunocytochemistry, Immunoprecipitation, Western Blot
Concentration
Please refer to lot specific datasheet.
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
O43151
Immunogen
KLH-conjugated linear peptide corrsponding to an internal sequence within the N-terminal half of human TET3.
Quantity
100 μg
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_001274420
Target Species
Mouse
Related Products
Description
- MABE1144/1132/1133 Methylcytosine dioxygenase TET3 (EC 1.14.11.n2; UniProt O43151) is encoded by the TET3 (also known as KIAA0401) gene (Gene ID 200424) in human
- TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), providing a means for active epigenetic DNA demethylation in mammals
- TET proteins also modify genomic thymine residues to 5-hydroxyuracil
- TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation
- In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins
- TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA
- High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported
- Three TET3 spliced isoforms/variants have been reported, the long or full-length isoform Tet3FL and the short isoform Tet3s are upregulated during embryonic stem cell (ESC) differentiation toward the neuronal lineage, while Tet3o is oocyte-specific and not detected in ESCs or in any other cell types or adult mouse tissues tested, indicating a role of Tet3o in paternal genome oxidation in mouse zygotes
- Tet3FL and Tet3o contain the entire Tet3s sequence with additional (but different) N-terminal sequence
- Only Tet3FL contains an N-terminal CXXC domain that binds to unmethylated CpGs and exhibits highest affinity toward 5caC
- Tet3FL is substantially less active than Tet3o and Tet3s, suggesting that the CXXC domain restricts Tet3FL 5mC oxidation capacity.
Compare Similar Items
Show Difference
Antigen: TET3
Classification: Polyclonal
Conjugate: Unconjugated
Gene: TET3, KIAA0401
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Immunogen sequence is present in TET3 isoforms Tet3FL, Tet3s, and Tet3o spliced isoforms reported by Jin, S.G., et al. (2016). Cell Rep. 14(3):493-505), as well as all additional human and mouse TET3 spliced isoforms reported by UniProt (O43151, Q8BG87). Note that UniProt isoform 1 (O43151-1/human and Q8BG87-1/mouse) corresponds to isoform Tet3s, not Tet3FL (NM_001287491.1/human and JX946278.1/mouse).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt. Note: It is recommended to store the product undiluted at 2°-8°C and protected from prolonged exposure to light. Do not freeze.
Applications: ChIP Assay, Immunocytochemistry, Immunoprecipitation, Western Blot
Concentration: Please refer to lot specific datasheet.
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.: O43151
Immunogen: KLH-conjugated linear peptide corrsponding to an internal sequence within the N-terminal half of human TET3.
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_001274420
Target Species: Mouse
Antigen:
TET3
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
TET3, KIAA0401
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Immunogen sequence is present in TET3 isoforms Tet3FL, Tet3s, and Tet3o spliced isoforms reported by Jin, S.G., et al. (2016). Cell Rep. 14(3):493-505), as well as all additional human and mouse TET3 spliced isoforms reported by UniProt (O43151, Q8BG87). Note that UniProt isoform 1 (O43151-1/human and Q8BG87-1/mouse) corresponds to isoform Tet3s, not Tet3FL (NM_001287491.1/human and JX946278.1/mouse).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt. Note: It is recommended to store the product undiluted at 2°-8°C and protected from prolonged exposure to light. Do not freeze.
Applications:
ChIP Assay, Immunocytochemistry, Immunoprecipitation, Western Blot
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.:
O43151
Immunogen:
KLH-conjugated linear peptide corrsponding to an internal sequence within the N-terminal half of human TET3.
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Gene ID (Entrez):
NP_001274420
Target Species:
Mouse
Antigen: Dcp2
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This rabbit polyclonal antibody detects m7GpppN-mRNA hydrolase (Dcp2) in human and murine cells.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications: Western Blot
Concentration: Please refer to lot specific datasheet.
Formulation: __
Gene Accession No.: __
Immunogen: His-tagged full length recombinant human m7GpppN-mRNA hydrolase (Dcp2).
Quantity: 100 μL
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_689837
Target Species: Mouse, Human
Antigen:
Dcp2
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Host Species:
Rabbit
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This rabbit polyclonal antibody detects m7GpppN-mRNA hydrolase (Dcp2) in human and murine cells.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications:
Western Blot
Concentration:
Please refer to lot specific datasheet.
Formulation:
__
Gene Accession No.:
__
Immunogen:
His-tagged full length recombinant human m7GpppN-mRNA hydrolase (Dcp2).
Quantity:
100 μL
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_689837
Target Species:
Mouse, Human
Antigen: Dcp2
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This rabbit polyclonal antibody detects m7GpppN-mRNA hydrolase (Dcp2) in human and murine cells.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications: Western Blot
Concentration: Please refer to lot specific datasheet.
Formulation: __
Gene Accession No.: __
Immunogen: His-tagged full length recombinant human m7GpppN-mRNA hydrolase (Dcp2).
Quantity: 25 μL
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_689837
Target Species: Mouse, Human
Antigen:
Dcp2
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Host Species:
Rabbit
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This rabbit polyclonal antibody detects m7GpppN-mRNA hydrolase (Dcp2) in human and murine cells.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications:
Western Blot
Concentration:
Please refer to lot specific datasheet.
Formulation:
__
Gene Accession No.:
__
Immunogen:
His-tagged full length recombinant human m7GpppN-mRNA hydrolase (Dcp2).
Quantity:
25 μL
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_689837
Target Species:
Mouse, Human
Antigen: __
Classification: __
Conjugate: __
Gene: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Test Specificity: __
Content And Storage: __
Applications: __
Concentration: __
Formulation: __
Gene Accession No.: __
Immunogen: __
Quantity: __
Research Discipline: __
Gene ID (Entrez): __
Target Species: __
Antigen:
__
Classification:
__
Conjugate:
__
Gene:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Test Specificity:
__
Content And Storage:
__
Applications:
__
Concentration:
__
Formulation:
__
Gene Accession No.:
__
Immunogen:
__
Quantity:
__
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
__