ABT1372
MilliporeSigma™ phospho-Keratin-8 (Ser23), Rabbit, Unlabeled, Polyclonal,
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABT1372-Each-of-1 | In Stock | ₹ 41,308.37 |
ABT1372 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,308.37
GST (18%): ₹ 7,435.507
Total Price: ₹ 48,743.877
Antigen
phospho-Keratin-8 (Ser23)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
KRT8, CYK8
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This rabbit polyclonal antibody detected the target phospho band only in lysates from phosphatase inhibitors-treated, but not untreated HeLa cells. Antibody blocking by the immunogen peptide, but not the corresponding nonphosphorylated peptide prior to immunoblotting abolished target band detection. The Ser23 designation is based on human keratin-8 sequence with initiator Met1 removed. The phosphorylation site sequence in mouse/rat (-SSR(pS)YT-) differs from that of human (-SSR(pS)FT-) by a single Phe24-to-Tyr24 switch (F24Y). Cross-reactivity of this polyclonal antibody toward murine and rat species is likely, but has not been confirmed.
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Applications
Western Blot
Concentration
Please refer to lot specific datasheet.
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
P05787
Immunogen
KLH-conjugated linear peptide corresponding to the phosphorylation site sequence containing the phosphorylated target serine.
Quantity
100 μL
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_002264
Target Species
Human
Related Products
Description
- Keratin, type II cytoskeletal 8 (UniProt P05787; also known as CK-8, Cytokeratin-8, K8, Keratin-8, Type-II keratin Kb8) is encoded by the KRT8 (also known as CYK8) gene (Gene ID 3856) in human
- Cytokeratin-8 (Keratin-8 or K8) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins that play an important role in the mechanical stability and integrity of epithelial cells and tissues
- Each epithelium is characterized by its unique keratin expression pattern
- Keratin 8 is a type II intermediate filament protein found in simple epithelia, where it pairs with keratin 18 (K8/KI8) and plays an important role in protecting cells from stress, injury, and apoptosis
- The structure and function of K8/18 are regulated by posttranslational modifications, including glycosylation, ubiquitination, and phosphorylation
- K8 has been reported to be phosphorylated on Ser8, Ser23, Ser73, and Ser431 by various protein kinases
- PKCepsilon is reported to mediate K8 phosphorylation on Ser8 and Ser23, p38 and PKCdelta are responsible for K8 Ser73 phosphorylation, while ERK and JNK are involved in K8 Ser431 phosphorylation.
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Antigen: phospho-Keratin-8 (Ser23)
Classification: Polyclonal
Conjugate: Unconjugated
Gene: KRT8, CYK8
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This rabbit polyclonal antibody detected the target phospho band only in lysates from phosphatase inhibitors-treated, but not untreated HeLa cells. Antibody blocking by the immunogen peptide, but not the corresponding nonphosphorylated peptide prior to immunoblotting abolished target band detection. The Ser23 designation is based on human keratin-8 sequence with initiator Met1 removed. The phosphorylation site sequence in mouse/rat (-SSR(pS)YT-) differs from that of human (-SSR(pS)FT-) by a single Phe24-to-Tyr24 switch (F24Y). Cross-reactivity of this polyclonal antibody toward murine and rat species is likely, but has not been confirmed.
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Applications: Western Blot
Concentration: Please refer to lot specific datasheet.
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.: P05787
Immunogen: KLH-conjugated linear peptide corresponding to the phosphorylation site sequence containing the phosphorylated target serine.
Quantity: 100 μL
Research Discipline: Cell Structure
Gene ID (Entrez): NP_002264
Target Species: Human
Antigen:
phospho-Keratin-8 (Ser23)
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
KRT8, CYK8
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This rabbit polyclonal antibody detected the target phospho band only in lysates from phosphatase inhibitors-treated, but not untreated HeLa cells. Antibody blocking by the immunogen peptide, but not the corresponding nonphosphorylated peptide prior to immunoblotting abolished target band detection. The Ser23 designation is based on human keratin-8 sequence with initiator Met1 removed. The phosphorylation site sequence in mouse/rat (-SSR(pS)YT-) differs from that of human (-SSR(pS)FT-) by a single Phe24-to-Tyr24 switch (F24Y). Cross-reactivity of this polyclonal antibody toward murine and rat species is likely, but has not been confirmed.
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Applications:
Western Blot
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.:
P05787
Immunogen:
KLH-conjugated linear peptide corresponding to the phosphorylation site sequence containing the phosphorylated target serine.
Quantity:
100 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_002264
Target Species:
Human
Antigen: Aggrecan
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This rabbit polyclonal antibody detects aggrecan in rat eye sections.
Content And Storage: Stable for 1 year at -20°C from date of receipt.
Applications: Immunohistochemistry, Immunohistochemistry (Paraffin), Radioimmune Assays (RIA), Western Blot
Concentration: __
Formulation: Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene Accession No.: __
Immunogen: GST-tagged recombinant fragment corresponding to 213 amino acids from the C-terminal half of rat Aggrecan.
Quantity: 25 μL
Research Discipline: Cell Structure
Gene ID (Entrez): __
Target Species: Rat
Antigen:
Aggrecan
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Host Species:
Rabbit
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This rabbit polyclonal antibody detects aggrecan in rat eye sections.
Content And Storage:
Stable for 1 year at -20°C from date of receipt.
Applications:
Immunohistochemistry, Immunohistochemistry (Paraffin), Radioimmune Assays (RIA), Western Blot
Concentration:
__
Formulation:
Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene Accession No.:
__
Immunogen:
GST-tagged recombinant fragment corresponding to 213 amino acids from the C-terminal half of rat Aggrecan.
Quantity:
25 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
__
Target Species:
Rat
Antigen: Acetyl Cortactin (Lys309)
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This polyclonal antibody specifically detects Src substrate cortactin acetylated on Lysine 309.
Content And Storage: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications: Immunofluorescence, Western Blot
Concentration: Please refer to lot specific datasheet.
Formulation: __
Gene Accession No.: __
Immunogen: KLH-conjugated linear peptide corresponding to 14 amino acids surrounding acetylated lysine309 of human Src substrate cortactin.
Quantity: 100 μL
Research Discipline: Cell Structure
Gene ID (Entrez): NP_005222
Target Species: Monkey
Antigen:
Acetyl Cortactin (Lys309)
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This polyclonal antibody specifically detects Src substrate cortactin acetylated on Lysine 309.
Content And Storage:
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications:
Immunofluorescence, Western Blot
Concentration:
Please refer to lot specific datasheet.
Formulation:
__
Gene Accession No.:
__
Immunogen:
KLH-conjugated linear peptide corresponding to 14 amino acids surrounding acetylated lysine309 of human Src substrate cortactin.
Quantity:
100 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_005222
Target Species:
Monkey
Antigen: __
Classification: __
Conjugate: __
Gene: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Test Specificity: __
Content And Storage: __
Applications: __
Concentration: __
Formulation: __
Gene Accession No.: __
Immunogen: __
Quantity: __
Research Discipline: __
Gene ID (Entrez): __
Target Species: __
Antigen:
__
Classification:
__
Conjugate:
__
Gene:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Test Specificity:
__
Content And Storage:
__
Applications:
__
Concentration:
__
Formulation:
__
Gene Accession No.:
__
Immunogen:
__
Quantity:
__
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
__