MilliporeSigma™ SET-beta, Rabbit, Unlabeled, Polyclonal,

Description

• Protein SET (UniProt Q9EQU5; also known as I-2PP2A, Phosphatase 2A inhibitor I2PP2A, TAF-I, Template-activating factor I) is encoded by the Set gene (Gene ID 56086) in murine species
• Protein SET (patient SE translocation) adopted its name when it was originally identified in a leukemia study in 1992
• SET interacts with signaling pathways that promote normal cell growth and proliferation, as well as tumorigenesis and metastasis
• SET protein inhibits PP2A by enhancing lyase activity of P450c17, a substrate for PP2A when it is Ser- and Thr-phosphorylated
• Alternative splicings result in multiple SET isoforms, including SET-alpha (TAF-I alpha), SET-beta (TAF-I beta), where the N-terminal 37-amino acid sequence in Set-alpha is replaced with a different sequence of a shorter length in Set-beta
• In addition, there exists a shorter spliced Set-beta isoform without the nuclear localization signal (NLS)
• Both Set-alpha and SET-beta are predominantly localized in the nucleus, but differ in their expression levels among various cell and tissue types
• SET-beta is resides predominantly in the brain relative to Set-alpha, whereas the opposite pattern was observed in tissues such as the spleen
• SET-beta functions have been described both in the nucleus and cytoplasm
• The presence of a nuclear localization signal (NLS) and phosphorylation govern SET-beta isoforms cellular localization
• Proteolytic cleavage of SET-beta at Lys176 by granzyme A or at Asn-175 by asparagine endopeptidase results in the release of GzmA-activated DNase (GAAD) from a multiprotein complex
• Uninhibited in the absence of the full-length Set-beta, GAAD causes cell death by its DNA-nicking activity.