SV40 Large T Antigen Mouse, Unlabeled, Clone: PAB 101, BD

Description

• Simian virus 40 is a small DNA virus encoded by 5.2 kb of double-stranded DNA
• SV40 large T antigen (T-ag) is a multifunctional ∽85kDa phosphoprotein, which is the sole viral protein required for SV40 replication
• All other factors are provided by the infected host cell
• In addition to its role in SV40 DNA replication, T-ag also causes transformation of susceptible cell lines
• Studies of various mutant T-ag proteins have shown that the replication and transformation fractions of T-ag can be separated
• The multifunctional nature of this protein has resulted in its use as a model system in a wide variety of disciplines
• SV40 T-ag exercises negative regulation on the transcription of SV40 early mRNA by feedback inhibition and exerts positive regulation on transcription from the late promoter
• In addition to transcriptional regulation, T-ag is involved in viral DNA replication
• Specific biochemical functions required for DNA synthesis that are inherent to the T-ag include high-affinity binding to sites within the viral origin of DNA synthesis, and ATPase and helicase activities
• Other functions attributed to T-ag include cellular transformation, induction of cellular DNA synthesis, induction of rRNA synthesis and provision of a host-range function for viral replication
• However, all functions of T-ag are influenced by a wide range of post-translational modifications including phosphorylation, glycosylation, acetylation, acylation and adenylation
• T-ag exists in monomeric as well as polymeric forms and associates with the tumor suppressor proteins p53 and Rb (retinoblastoma protein)
• Most of T-ag is transported to the nucleus, while a small fraction is localized at the cell surface
• PAb 101 recognizes a C-terminal epitope within the last 190 amino acids of T-ag
• PAb 101 was originally known as Clone 7
• Studies have suggested that PAb 101 binds the strongest to mature T-ag
• PAb 101 (i.e., Clone 7) was developed along with a panel of monoclonal antibodies where SV40-transformed BALB/c mouse cell lines (SVT2 or B4) were used as immunogens
• The specificity of the antibody was originally characterized by a variety of techniques using SV40-infected and SV40-transformed cells
• Host Species: Mouse Clone: PAb 101 Isotype: IgG2a Species Reactivity: Viral-transformed cells Immunogen: SV40-transformed BALB/c mouse cell lines Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting