SV40 Large T Antigen Mouse, Unlabeled, Clone: PAB 100, BD

Description

• Simian virus 40 is a small DNA virus encoded by 5.2 kb of double-stranded DNA
• SV 40 large T antigen (T-ag) is a multifunctional 85kda phosphoprotein, which is the sole viral protein required for SV40 replication
• All other factors are provided by the infected host cell
• In addition to its role in SV40 DNA replication, T-ag also causes transformation of susceptible cell lines
• Studies of various mutant T-ag proteins have shown that the replication and transformation fractions of T-ag can be separated
• The multifunctional nature of this protein has resulted in its use as a model system in a wide variety of disciplines
• SV40 T-ag exercises negative regulation on the transcription of SV40 early mRNA by feedback inhibition and exerts positive regulation on transcription from the late promoter
• In addition to transcriptional regulation, T-ag is involved in viral DNA replication
• Specific biochemical functions required for DNA synthesis that are inherent to the T-ag include high-affinity binding to sites within the viral origin of DNA synthesis and ATPase and helicase activities
• Other functions attributed to T-ag include cellular transformation, induction of cellular DNA synthesis, induction of rRNA synthesis, and provision of a host-range function for viral replication
• However, all functions of T-ag are influenced by a wide range of post-translational modifications including phosphorylation, glycosylation, acetylation, acylation, and adenylation
• T-ag exists in monomeric as well as polymeric forms and associates with the tumor suppressor proteins p53 and Rb (retinoblastoma protein)
• Most of T-ag is transported to the nucleus, while a small fraction is localized at the cell surface
• PAb 100 recognizes an epitope between amino acids 270 and 520 of T-ag
• PAb 100 was originally known as clone 412
• Studies have suggested that PAb 100 binds the strongest to newly synthesized T-ag and to ATPase-active T-ag in some experimental systems
• In other experimental systems it appears to preferentially bind to recognize mature T-ag
• PAb 100 (i.e., clone 412) was developed along with a panel of monoclonal antibodies where SV40-transformed BALB/c mouse cell lines (SVT2 or B4) were used as immunogens
• The specificity of the antibody was originally characterized by a variety of techniques using SV40-infected and SV40-transformed cells
• Host Species: Mouse Clone: PAb 100 Isotype: IgG1 Species Reactivity: Viral-transformed cells Immunogen: SV40-transformed BALB/c mouse cell lines Formula Weight [Chemical]: 85-100kDa Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation