SV40 Large T and Small T Antigens Mouse, Unlabeled, Clone: PAB 108, BD

Description

• Simian virus 40 (SV40) is a small DNA virus encoded by 5.2 kb of double-stranded DNA
• SV40 large T-antigen (T-ag) is a multifunctional ∽85kDa phosphoprotein, which is the sole viral protein required for SV40 replication
• All other factors are provided by the infected host cell
• In addition to its role in SV40 DNA replication, T-ag also causes transformation of susceptible cell lines
• Studies of various mutant T-ag proteins have shown that the replication and transformation fractions of T-ag can be separated
• The multifunctional nature of this protein has resulted in its use as a model system in a wide variety of disciplines
• T-ag exercises negative regulation on the transcription of SV40 early mRNA by feedback inhibition and exerts positive regulation on transcription from the late promoter
• In addition to transcriptional regulation, T-ag is involved in viral DNA replication
• Specific biochemical functions required for DNA synthesis that are inherent to the T-ag include high-affinity binding to sites within the viral origin of DNA synthesis, ATPase, and helicase activities
• Other functions attributed to T-ag include cellular transformation, induction of cellular DNA synthesis, induction of rRNA synthesis, and provision of a host-range function for viral replication
• However, functions of T-ag are influenced by a wide range of post-translational modifications including phosphorylation, glycosylation, acetylation, acylation, and adenylation
• T-ag exists in monomeric as well as polymeric forms, and associates with the tumor suppressor proteins p53 and retinoblastoma protein (Rb)
• Most of T-ag is transported to the nucleus, while a small fraction is localized at the cell surface
• Small t-Ag is a polypeptide which shares 82 N-terminal amino acids with large T antigen and has a unique C-terminal region
• Clone PAb 108 recognizes an N-terminal epitope within the first 82 amino acids of T-ag and small t antigen (t-ag)
• B4 SV40-transformed BALB/c mouse fibroblasts were used as immunogen
• PAb 108 was originally produced and characterized as part of a panel of antibodies designated PAb 102-117
• Host Species: Mouse Clone: PAb 108 Isotype: IgG2a Species Reactivity: Viral-transformed cells Immunogen: SV40-transformed BALB/c mouse cells Formula Weight [Chemical]: 90-100kDa Immunofluorescence, Immunoprecipitation, Western Blotting