Adenovirus Type 5 E1A Mouse anti-Human, Unlabeled, Clone: M58, BD

Description

• Human adenoviruses are widely used as models for studying cellular transformation and regulation of gene expression
• The adenovirus E1A region plays a central role in each of these processes
• This region of the viral genome encodes a series of related proteins with multifunctional capabilities
• E1A regulates the transcriptional regulation of a wide variety of viral and cellular promoters
• Most commonly, this results in the activation of transcription from the target promoter
• In other cases, transcription from the targeted promoter is repressed by the action of E1A
• E1A proteins also contribute to the transforming capabilities of adenoviruses
• The E1A and E1B regions together comprise the transforming region of adenovirus
• While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region
• Several other oncogenes, including the activated H-ras oncogene and polyoma middle T antigen, can complement E1A by substituting for E1B in transformation assays
• Similarly, E1A can be replaced by polyoma large T antigen, c-myc and mutated p53 protein
• Several conserved regions of E1A are strikingly similar to portions of other viral oncoproteins, e.g., HPV-16, HPV-18 E7 and SV40 large T antigen
• Like HPV E7 and SV40 large T antigen, E1A proteins can form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein)
• Complex formation between the products of oncogenes and tumor suppressor genes are believed to be important in cellular transformation
• The exact mechanism(s) of these complexes are not fully understood, however they may be involved in negative regulation of cellular growth and/or differentiation
• M58 recognizes Adenovirus Type 5 E1A proteins.4 A trpE-E1A fusion protein was used as immunogen
• The fusion protein contained the C-terminal 266 amino acids of the 13S E1A protein
• This is about 90% of the E1A 13S coding sequence.