PKCθ (pT538) Mouse anti-Human, Unlabeled, Clone: 19, BD

Description

• The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion
• Three categories exist, conventional PKC (cPKC), novel PKC (nPKC), and atypical PKC (aPKC)
• These proteins are products of multiple genes and alternative splicing and have different modes of activation
• For example, cPKC's members (α, βI, βII, and γ) are calcium activated, phospholipid-dependent serine/threonine specific enzymes which can also be activated by phorbol esters
• However, the novel PKC (nPKC) subfamily members (δ , ε, π, and θ isoforms) and the atypical PKC (PKC) subfamily members (ζ , f , andλ isoforms) are Ca 2+ ; independent
• The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters
• The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors
• PKCθ transcripts are expressed in most tissues with the highest levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes
• PKCθ RNA is readily detectable in skeletal muscle, lung, and brain
• However, PKCθ expression is not detected in several human carcinoma cell lines
• Abundant expression of this PKC isozyme in hematopoietic cells suggests that it may have a role in growth and differentiation processes of these cells
• The 9/PKC monoclonal antibody recognizes the phosphorylated threonine 538 (pT538) of human PKCθ
• Western Blotting