FcγRIV (CD16-2) Hamster anti-Mouse, Brilliant Violet 605, Clone: 9E9., BD Optibuild™

Description

• The 9E9 monoclonal antibody specifically binds to Fc receptor, IgG, low affinity IV (FcγRIV), which is also known as CD16-2
• FcγRIV is encoded by Fcgr4 /and belongs to the family of receptors for the Fc region of IgG (FcγRs), which also includes FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16) within the Ig superfamily.FcγRIVis expressed on monocytes, macrophages, dendritic cells, and neutrophils.FcγRIV, which serves as a ligand-biding subunit, requiresthe common Fcγ chain for expression and signaling
• This complex serves as a cell activating receptor when bound by IgG2a or IgG2b
• FcγRIV plays various roles in inflammation including neutrophil trafficking and mast cell degranulation.FcγRIVcan also function as a low-affinity IgE receptor and promote IgE-induced inflammation This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.